PUBLICATION
Cloning and characterization of two novel zebrafish P2X receptor subunits
- Authors
- Diaz-Hernandez, M., Cox, J.A., Migita, K., Haines, W., Egan, T.M., and Voigt, M.M
- ID
- ZDB-PUB-020912-20
- Date
- 2002
- Source
- Biochemical and Biophysical Research Communications 295(4): 849-853 (Journal)
- Registered Authors
- Voigt, Mark
- Keywords
- ionotropic; receptor; ligand-gated channel; purinoceptor; nucleotide; zebrafish; ATP
- MeSH Terms
-
- Adenosine Triphosphate/metabolism
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary/metabolism
- Dose-Response Relationship, Drug
- Electrophysiology
- Humans
- Ligands
- Molecular Sequence Data
- Physical Chromosome Mapping
- Protein Structure, Tertiary
- Receptors, Purinergic P2/chemistry*
- Receptors, Purinergic P2/genetics*
- Receptors, Purinergic P2X4
- Receptors, Purinergic P2X5
- Transfection
- Zebrafish
- PubMed
- 12127972 Full text @ Biochem. Biophys. Res. Commun.
Citation
Diaz-Hernandez, M., Cox, J.A., Migita, K., Haines, W., Egan, T.M., and Voigt, M.M (2002) Cloning and characterization of two novel zebrafish P2X receptor subunits. Biochemical and Biophysical Research Communications. 295(4):849-853.
Abstract
In this report we describe the cloning and characterization of two P2X receptor subunits cloned from the zebrafish (Danio rerio). Primary sequence analysis suggests that one cDNA encodes an ortholog of the mammalian P2X(4) subunit and the second cDNA encodes the ortholog of the mammalian P2X(5) subunit. The zP2X(4) subunit forms a homo-oligomeric receptor that displays a low affinity for ATP (EC(50)=274+/-48 microM) and very low affinity (EC(50)>500 microM) for other purinergic ligands such as alphabetameATP, suramin, and PPADS. As seen with the mammalian orthologs, the zP2X(5) subunit forms a homo-oligomeric receptor that yields very small whole-cell currents (<20pA), making determination of an EC(50) problematic. Both subunit genes were physically mapped onto the zebrafish genome using radiation hybrid analysis of the T51 panel, with the zp2x4 localized to LG21 and zp2x5 to LG5.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping