| ZFIN ID: ZDB-PUB-020912-2 |
BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain
Mueller, T., and Wullimann, M.
| Date: | 2002 |
|---|---|
| Source: | Mechanisms of Development 117(1-2): 123-135 (Journal) |
| Registered Authors: | Mueller, Thomas, Wullimann, Mario F. |
| Keywords: | zebrafish; Hu-protein; 5-bromo-2'-deoxyuridine; proliferating cell nuclear antigen; neuroD; nrd; forebrain; diencephalon; preglomerular area; pretectum; posterior tuberculum; telencephalon; cerebellum; external granular layer; basic helix-loop-helix transcription; factor; mitosis; proliferation |
| MeSH Terms: |
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| PubMed: | 12204253 Full text @ Mech. Dev. |
Citation
Mueller, T., and Wullimann, M. (2002) BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain. Mechanisms of Development. 117(1-2):123-135.
ABSTRACT
In the postembryonic zebrafish forebrain, subpial locations of neurogenesis do exist in the early cerebellar external granular layer, and - unusually among vertebrates - in the primordial pretectal (M1) and preglomerular (M2) Anlagen as shown here with 5-bromo-2'-deoxyuridine ( BrdU)/Hu-immunocytochemistry and in situ hybridization of neuroD. An intermediate BrdU incubation time of 12-16h reveals in addition to proliferative ventricularly located cells those in M1 and M2. This BrdU saturation-labeling shows - in conjunction with a Hu-assay demonstrating earliest neuronal differentiation - that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU- labeled and double BrdU-/Hu-labeled cells in these aggregates. Further, expression of NeuroD - a marker for freshly determined neuronal cells - confirms this unusual subpial postembryonic forebrain neurogenesis.
ADDITIONAL INFORMATION
- Genes / Markers (2)
- Antibodies (1)
- Fish (1)