PUBLICATION
BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain
- Authors
- Mueller, T., and Wullimann, M.
- ID
- ZDB-PUB-020912-2
- Date
- 2002
- Source
- Mechanisms of Development 117(1-2): 123-135 (Journal)
- Registered Authors
- Mueller, Thomas, Wullimann, Mario F.
- Keywords
- zebrafish; Hu-protein; 5-bromo-2'-deoxyuridine; proliferating cell nuclear antigen; neuroD; nrd; forebrain; diencephalon; preglomerular area; pretectum; posterior tuberculum; telencephalon; cerebellum; external granular layer; basic helix-loop-helix transcription; factor; mitosis; proliferation
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors
- Bromodeoxyuridine/metabolism*
- Cell Differentiation
- Cell Division
- ELAV Proteins
- Immunohistochemistry
- In Situ Hybridization
- Larva/cytology
- Larva/growth & development
- Larva/metabolism
- Mitosis
- Nerve Tissue Proteins/genetics*
- Neurons/cytology
- Neurons/metabolism
- Proliferating Cell Nuclear Antigen/metabolism
- Prosencephalon/cytology
- Prosencephalon/growth & development*
- Prosencephalon/metabolism*
- RNA-Binding Proteins/metabolism*
- Stem Cells/cytology
- Stem Cells/metabolism
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism*
- PubMed
- 12204253 Full text @ Mech. Dev.
Citation
Mueller, T., and Wullimann, M. (2002) BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain. Mechanisms of Development. 117(1-2):123-135.
Abstract
In the postembryonic zebrafish forebrain, subpial locations of neurogenesis do exist in the early cerebellar external granular layer, and - unusually among vertebrates - in the primordial pretectal (M1) and preglomerular (M2) Anlagen as shown here with 5-bromo-2'-deoxyuridine ( BrdU)/Hu-immunocytochemistry and in situ hybridization of neuroD. An intermediate BrdU incubation time of 12-16h reveals in addition to proliferative ventricularly located cells those in M1 and M2. This BrdU saturation-labeling shows - in conjunction with a Hu-assay demonstrating earliest neuronal differentiation - that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU- labeled and double BrdU-/Hu-labeled cells in these aggregates. Further, expression of NeuroD - a marker for freshly determined neuronal cells - confirms this unusual subpial postembryonic forebrain neurogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping