|ZFIN ID: ZDB-PUB-020912-17|
Furrow-specific endocytosis during cytokinesis of zebrafish blastomeres
Feng, B., Schwarz, H., and Jesuthasan, S.
|Source:||Experimental cell research 279(1): 14-20 (Journal)|
|Registered Authors:||Feng, Bo, Jesuthasan, Suresh|
|Keywords:||cytokinesis; endocytosis; clathrin; caveolin; Danio rerio|
|PubMed:||12213209 Full text @ Exp. Cell Res.|
Feng, B., Schwarz, H., and Jesuthasan, S. (2002) Furrow-specific endocytosis during cytokinesis of zebrafish blastomeres. Experimental cell research. 279(1):14-20.
ABSTRACTMutations affecting endocytosis, such as those in clathrin and dynamin, unexpectedly cause defects in cytokinesis in a number of organisms. To explore the relationship between endocytosis and cytokinesis, we used the relatively large cells of the transparent zebrafish embryo. Using fluorescent markers for fluid-phase as well as plasma membrane uptake, we demonstrate that cytokinesis involves furrow-specific endocytosis. Clathrin-coated pits are visible near the furrow in ultrathin sections, while immunolabeling demonstrates that clathrin and caveolin are localized to the cleavage furrow. Hence, it is likely that both clathrin - and caveolae-mediated endocytosis occurs at the furrow during cytokinesis. Dynamin II is also localized to the furrow and may mediate furrow-specific endocytosis. Treatment of embryos with chlorpromazine or with methyl-beta-cyclodextrin, both of which inhibit endocytosis, prevents the normal completion of cytokinesis. These data suggest that furrow-specific endocytosis is an integral part of cytokinesis.
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