PUBLICATION
Construction of a zebrafish cDNA microarray: gene expression profiling of the zebrafish during development
- Authors
- Ton, C., Stamatiou, D., Dzau, V., and Liew, C.
- ID
- ZDB-PUB-020912-12
- Date
- 2002
- Source
- Biochemical and Biophysical Research Communications 296(5): 1134-1142 (Journal)
- Registered Authors
- Liew, Choong-Chin, Ton, Christopher
- Keywords
- zebrafish; Danio rerio; cDNA microarray; gene expression; development; hypoxia
- MeSH Terms
-
- Animals
- Cell Hypoxia
- Cells, Cultured
- Embryo, Nonmammalian/metabolism
- Gene Expression Profiling/methods
- Kinetics
- Oligonucleotide Array Sequence Analysis/methods
- RNA, Messenger/biosynthesis
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 12207891 Full text @ Biochem. Biophys. Res. Commun.
Citation
Ton, C., Stamatiou, D., Dzau, V., and Liew, C. (2002) Construction of a zebrafish cDNA microarray: gene expression profiling of the zebrafish during development. Biochemical and Biophysical Research Communications. 296(5):1134-1142.
Abstract
Vertebrate embryogenesis is a complex process controlled by a transcriptional hierarchy that coordinates the action of thousands of genes. To identify and analyze the expression patterns of these genes, we constructed a zebrafish cDNA microarray containing 4512 unique genes identified from zebrafish embryonic heart, adult hearts, and skeletal muscle cDNA libraries. We examined the patterns of gene expression during development in the zebrafish between five time points relative to 12h post-fertilization (hpf). Differentially expressed genes can be grouped into two categories, early genes that are expressed at 5hpf and genes expressed at 48/72/120hpf. Furthermore, we report the utilization of cDNA microarray technology to investigate the adaptive molecular responses of zebrafish to hypoxia during development. Our study provides the first utilization of cDNA microarray in the zebrafish and reveals dynamic changes in levels of gene expression in relation to development and survival of the zebrafish embryos under hypoxic stress.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping