PUBLICATION
Type I keratin cDNAs from the rainbow trout: independent radiation of keratins in fish
- Authors
- Schaffeld, M., Höffling, S., Haberkamp, M., Conrad, M. and Markl, J.
- ID
- ZDB-PUB-020904-7
- Date
- 2002
- Source
- Differentiation; research in biological diversity 70(6): 282-291 (Journal)
- Registered Authors
- Conrad, Matthias
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal/immunology
- Antibody Specificity
- Cloning, Molecular
- DNA, Complementary/genetics
- Epidermis/metabolism
- Epithelial Cells/metabolism
- Evolution, Molecular*
- Humans
- Keratins/classification
- Keratins/genetics*
- Keratins/immunology
- Lampreys/genetics
- Mammals/genetics
- Mesoderm/metabolism
- Molecular Sequence Data
- Oncorhynchus mykiss/genetics*
- Organ Specificity
- Peptide Mapping
- Phylogeny
- Protein Isoforms/genetics
- Protein Isoforms/immunology
- Protein Structure, Tertiary
- Sequence Alignment
- Sequence Homology, Amino Acid
- Sharks/genetics
- Species Specificity
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Zebrafish/genetics
- PubMed
- 12190989 Full text @ Differentiation
Citation
Schaffeld, M., Höffling, S., Haberkamp, M., Conrad, M. and Markl, J. (2002) Type I keratin cDNAs from the rainbow trout: independent radiation of keratins in fish. Differentiation; research in biological diversity. 70(6):282-291.
Abstract
Five different type I keratins from a teleost fish, the rainbow trout Oncorhynchus mykiss, have been sequenced by cDNA cloning and identified at the protein level by peptide mass mapping using MALDI-MS. This showed that the entire range of type I keratins detected biochemically in this fish has now been sequenced. Three of the keratins are expressed in the epidermis (subtype Ie), whereas the other two occur in simple epithelia and mesenchymal cells (subtype Is). Among the Is keratins is an ortholog of human K18; the second Is polypeptide is clearly distinct from K18. We raised a new monoclonal antibody (F1F2, subclass IgG1) that specifically recognizes trout Is keratins, with negative reactions on zebrafish. A phylogenetic tree has been constructed from a multiple alignment of the rod domains of the new sequences together with type I sequences from other vertebrates such as shark, zebrafish, and human; a recently sequenced lamprey Is keratin was applied as outgroup. This tree shows one branch defining the K18 orthologs and a second branch containing all other type I keratins (mostly subtype Ie). Within this second branch, the teleost keratins form a separate, highly bootstrap- supported twig. This tree leaves little doubt that the teleost Ie keratins diversified independently from the mammalian Ie keratins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping