ZFIN ID: ZDB-PUB-020807-11
Cloned zebrafish by nuclear transfer from long-term-cultured cells
Lee, K-Y., Huang, H., Ju, B., Yang, Z., and Lin, S.
Date: 2002
Source: Nat. Biotechnol.   20(8): 795-799 (Journal)
Registered Authors: Huang, Haigen, Ju, Bensheng, Lee, Ki-Young, Lin, Shuo, Yang, Zhongan
Keywords: none
MeSH Terms:
  • Animals
  • Cells, Cultured
  • Chromosomes/genetics
  • Cloning, Organism/methods*
  • Embryo, Nonmammalian/embryology*
  • Embryonic Development
  • Female
  • Fibroblasts
  • Male
  • Nuclear Transfer Techniques*
  • Ovum/cytology
  • Time Factors
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/growth & development
PubMed: 12134167 Full text @ Nat. Biotechnol.
Although mammals have been cloned from genetically manipulated cultured cells, a comparable achievement has not been realized in lower vertebrates. Here we report that fertile transgenic zebrafish can be obtained by nuclear transfer using embryonic fibroblast cells from long- term cultures. The donor nuclei, modified by retroviral insertions expressing green fluorescent protein (GFP), were transplanted into manually enucleated eggs. Overall, a 2% success rate was achieved, resulting in 11 adult transgenic zebrafish expressing GFP. These nuclear transplants produced fertile, diploid offspring, and their F1/F2 progeny continued to express GFP in a pattern identical to that of the founder fish. This finding demonstrates that slowly dividing nuclei from cultured cells can be reprogrammed to support rapid embryonic development and sets up a foundation for targeted genetic manipulation in zebrafish.