PUBLICATION
Mammalian germ-line transgenesis by transposition
- Authors
- Dupuy, A.J., Clark, K., Carlson, C.M., Fritz, S., Davidson, A.E., Markley, K.M., Finley, K., Fletcher, C.F., Ekker, S.C., Hackett, P.B., Horn, S., and Largaespada, D.A.
- ID
- ZDB-PUB-020805-4
- Date
- 2002
- Source
- Proceedings of the National Academy of Sciences of the United States of America 99(7): 4495-4499 (Journal)
- Registered Authors
- Clark, Karl, Ekker, Stephen C., Hackett, Perry B.
- Keywords
- none
- MeSH Terms
-
- DNA Transposable Elements*
- Transposases/genetics
- Transposases/physiology
- Molecular Sequence Data
- Base Sequence
- Cloning, Molecular
- Animals
- Blotting, Southern
- Mutagenesis, Insertional*
- PubMed
- 11904379 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Dupuy, A.J., Clark, K., Carlson, C.M., Fritz, S., Davidson, A.E., Markley, K.M., Finley, K., Fletcher, C.F., Ekker, S.C., Hackett, P.B., Horn, S., and Largaespada, D.A. (2002) Mammalian germ-line transgenesis by transposition. Proceedings of the National Academy of Sciences of the United States of America. 99(7):4495-4499.
Abstract
Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called Sleeping Beauty in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping