PUBLICATION
Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A
- Authors
- Hannus, M., Feiguin, F., Heisenberg, C.-P., and Eaton, S.
- ID
- ZDB-PUB-020716-22
- Date
- 2002
- Source
- Development (Cambridge, England) 129(14): 3493-3503 (Journal)
- Registered Authors
- Heisenberg, Carl-Philipp
- Keywords
- Drosophila; Widerborst; PP2A; zebrafish; planar polarity
- MeSH Terms
-
- Genes, Insect
- Protein Phosphatase 2/genetics
- Protein Phosphatase 2/physiology*
- Cell Polarity/genetics
- Cell Polarity/physiology
- Gastrula/enzymology
- Wings, Animal/cytology
- Wings, Animal/growth & development
- Frizzled Receptors
- Mutation
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Adaptor Proteins, Signal Transducing
- Animals
- Cadherins/genetics
- Body Patterning/genetics
- Body Patterning/physiology
- Male
- Microtubules/enzymology
- Drosophila/cytology
- Drosophila/enzymology*
- Drosophila/genetics
- Drosophila/growth & development*
- Phosphoproteins/genetics
- Membrane Proteins/genetics
- Drosophila Proteins/genetics
- Drosophila Proteins/physiology*
- Receptors, G-Protein-Coupled
- Hair/growth & development
- Female
- Phenotype
- PubMed
- 12091318 Full text @ Development
Citation
Hannus, M., Feiguin, F., Heisenberg, C.-P., and Eaton, S. (2002) Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A. Development (Cambridge, England). 129(14):3493-3503.
Abstract
We have identified widerborst (wdb), a B' regulatory subunit of PP2A, as a conserved component of planar cell polarization mechanisms in both Drosophila and in zebrafish. In Drosophila, wdb acts at two steps during planar polarization of wing epithelial cells. It is required to organize tissue polarity proteins into proximal and distal cortical domains, thus determining wing hair orientation. It is also needed to generate the polarized membrane outgrowth that becomes the wing hair. Widerborst activates the catalytic subunit of PP2A and localizes to the distal side of a planar microtubule web that lies at the level of apical cell junctions. This suggests that polarized PP2A activation along the planar microtubule web is important for planar polarization. In zebrafish, two wdb homologs are required for convergent extension during gastrulation, supporting the conjecture that Drosophila planar cell polarization and vertebrate gastrulation movements are regulated by similar mechanisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping