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ZFIN ID: ZDB-PUB-020618-8
pc1 and psc1, zebrafish homologs of Drosophila Polycomb and Posterior sex combs, encode nuclear proteins capable of complex interactions
Kawamura, A., Yokota, S., Yamada, K., Inoue, H., Inohaya, K., Yamazaki, K., Yasumasu, I., and Higashinakagawa, T.
Date: 2002
Source: Biochemical and Biophysical Research Communications   294(2): 456-463 (Journal)
Registered Authors: Inohaya, Keiji, Kawamura, Akinori
Keywords: polycomb group; zebrafish; gene expression; nuclear complex; protein-protein interactions
MeSH Terms:
  • Animals
  • Binding Sites/physiology
  • Blastomeres/cytology
  • Blastomeres/metabolism
  • Blotting, Northern
  • Cell Nucleus/metabolism
  • DNA-Binding Proteins*
  • Drosophila
  • Drosophila Proteins*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • In Situ Hybridization
  • Insect Proteins/genetics*
  • Insect Proteins/metabolism
  • Molecular Sequence Data
  • Nuclear Proteins/genetics*
  • Nuclear Proteins/metabolism*
  • Polycomb Repressive Complex 1
  • Protein Binding/physiology
  • RNA, Messenger/biosynthesis
  • Two-Hybrid System Techniques
  • Zebrafish/embryology
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 12051733 Full text @ Biochem. Biophys. Res. Commun.
Drosophila Polycomb group proteins are thought to form multimeric nuclear complexes that are responsible for stable transmission of repressed states of gene expression during the proliferation of differentiating embryos. In this study, we cloned, sequenced, and characterized two Polycomb group homologs, designated pc1 and psc1, in zebrafish. Amino acid sequence analyses determined that pc1 is a structural homolog of Drosophila Polycomb and that psc1 is a homolog of Drosophila Posterior sex combs. Northern blots and whole-mount in situ hybridization revealed that pc1 and psc1 had overlapping expression patterns at successive stages of embryogenesis. Immunocytochemistry localized both Pc1 and Psc1 protein in blastomere nuclei. Pull-down assays and two-hybrid system deletion analyses showed that these proteins were capable of homotypic and heterotypic interactions and identified the regions required for these interactions. The evidence supports the idea that zebrafish Polycomb group proteins, like those of other species, form nuclear complexes with compositions that may vary in a spatio-temporal manner during development.