PUBLICATION
PCNA in the ovary of zebrafish (Brachydanio rerio, Ham.-Buch.)
- Authors
- Korfsmeier, K.H.
- ID
- ZDB-PUB-020514-5
- Date
- 2002
- Source
- Acta histochemica 104(1): 73-76 (Journal)
- Registered Authors
- Keywords
- PCNA; oocytes; follicle epithelium; oocyte degeneration; gen amplification; zebrafish
- MeSH Terms
-
- Animals
- Cell Nucleus/chemistry
- Female
- Fluorescent Antibody Technique, Indirect
- Meiosis
- Oocytes/chemistry*
- Oocytes/cytology
- Ovarian Follicle/chemistry*
- Ovarian Follicle/cytology
- Ovarian Follicle/growth & development
- Proliferating Cell Nuclear Antigen/analysis*
- Zebrafish
- PubMed
- 11993853 Full text @ Acta Histochem.
Citation
Korfsmeier, K.H. (2002) PCNA in the ovary of zebrafish (Brachydanio rerio, Ham.-Buch.). Acta histochemica. 104(1):73-76.
Abstract
PCNA, a nuclear protein intimately involved in DNA synthesis, has been localized immunohistochemically in ovaries of zebrafish (Brachydanio rerio, Ham.-Buch.). Positively stained cells were detected in the follicle epithelium of all stages of growing oocytes and degenerating oocytes. Furthermore, PCNA was found to be present in nuclei of oogonia and oocytes in meiotic prophase. Follicle epithelium showed an increasing number of PCNA-positive cells during egg cell development with a very low index of <0.5% at the beginning and a maximum of >7% in stage IV oocytes that are rapidly incorporating yolk protein. In this phase, follicle epithelium often showed groups of stained cells in limited zones of proliferation. In degenerating oocytes, over 60% of the former follicle cells were positive for PCNA. Nuclei of oogonia were always positive and the nucleus of stage III oocytes showed strong positivity. Stage III is the stage in which amplification of rRNA genes occurs and numerous nucleoli apear in the peripheral caryoplasm. It is concluded that PCNA expression is related to amplification of rDNA.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping