PUBLICATION
Vitellogenin induction by 17beta-estradiol and 17alpha-ethinylestradiol in male zebrafish (Danio rerio)
- Authors
- Rose, J., Holbech, H., Lindholst, C., Narum, U., Povlsen, A., Korsgaard, B., and Bjerregaard, P.
- ID
- ZDB-PUB-020513-9
- Date
- 2002
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 131(4): 531-539 (Journal)
- Registered Authors
- Bjerregaard, Poul, Holbech, Henrik, Korsgaard, Bodil, Lindholst, Christian
- Keywords
- EC50; ELISA; endocrine disrupters; 17b-estradiol; 17a-ethinylestradiol; LC-MS; rainbow trout; vitellogenin; zebrafish
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Estradiol/analysis
- Estradiol/pharmacology*
- Ethinyl Estradiol/analysis
- Ethinyl Estradiol/pharmacology*
- Logistic Models
- Male
- No-Observed-Adverse-Effect Level
- Vitellogenins/biosynthesis*
- Water/analysis
- Zebrafish
- PubMed
- 11976068 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Rose, J., Holbech, H., Lindholst, C., Narum, U., Povlsen, A., Korsgaard, B., and Bjerregaard, P. (2002) Vitellogenin induction by 17beta-estradiol and 17alpha-ethinylestradiol in male zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 131(4):531-539.
Abstract
Adult male zebrafish (Danio rerio) were exposed to 17beta-estradiol (E2) or 17alpha-ethinylestradiol (EE2) in flow-through systems for 8 days. This was done to compare the sensitivity of the estrogen inducible vitellogenin (Vtg) biomarker system of this proposed OECD test guideline species to other relevant test species. Vtg was quantified in whole body homogenate by a species-specific ELISA. Actual water concentrations of E2 and EE2 were quantified by LC-MS, with detection limits of 1.0 and 0.6 ng/l, respectively. Vtg induction (LOEC) occurred in whole body homogenate at actual water concentrations of 21 ng E2/l and 3.0 ng EE2/l, respectively. As an alternative to the ANOVA approach, the relationship between the percentage of responding fish (Vtg) and the external E2 or EE2 concentration was determined by logistic regression analysis. Based on the regression analysis, EC-values could be determined: EC(10), EC(50) and EC(90) were 15.4, 41.2 and 67.1 ng E2/l, respectively and 0.92, 2.51 and 4.09 ng EE2/l, respectively. Comparisons of these response limits to corresponding values for rainbow trout (Oncorhynchus mykiss), fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes) revealed the zebrafish as a sensitive test species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping