PUBLICATION
Regulation of alternative splicing of alpha-actinin transcript by Bruno-like proteins
- Authors
- Suzuki, H., Jin, Y., Otani, H., Yasuda, K., and Inoue, K.
- ID
- ZDB-PUB-020402-2
- Date
- 2002
- Source
- Genes to cells : devoted to molecular & cellular mechanisms 7(2): 133-141 (Journal)
- Registered Authors
- Inoue, Kunio, Suzuki, Hiroaki
- Keywords
- none
- MeSH Terms
-
- Actinin/genetics*
- Alternative Splicing*
- Amino Acid Motifs/genetics
- Animals
- CELF1 Protein
- Dinucleotide Repeats/genetics
- ELAV Proteins
- ELAV-Like Protein 3
- Gene Expression Regulation
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- RNA-Binding Proteins/genetics*
- RNA-Binding Proteins/metabolism
- Zebrafish
- Zebrafish Proteins*
- PubMed
- 11895477 Full text @ Genes Cells
Citation
Suzuki, H., Jin, Y., Otani, H., Yasuda, K., and Inoue, K. (2002) Regulation of alternative splicing of alpha-actinin transcript by Bruno-like proteins. Genes to cells : devoted to molecular & cellular mechanisms. 7(2):133-141.
Abstract
The Bruno-like or CELF proteins, such as mammalian CUGBP1 and Etr-3, Xenopus EDEN-BP, and Drosophila Bruno (Bru), are regulators of gene expression at the post-transcriptional level, and contain three RNA-recognition motifs (RRMs). It has been shown that mammalian CUGBP1 and Etr-3 regulate alternative splicing of cardiac troponin T pre-mRNA via binding to CUG-triplet repeats. RESULTS: Using in vitro selection and UV-crosslinking experiments, we found that zebrafish Bruno-like proteins bound to repeat elements of uridine and purine (termed UREs). It is known that non-muscle (NM) and smooth muscle (SM) exons of the rat alpha-actinin gene are used in a mutually exclusive manner. Transfection experiments in mammalian cells showed that zebrafish Brul and Etr-3 induced the muscle-specific splicing of rat alpha-actinin pre-mRNA via binding to the URE at the branch point upstream of the NM exon. In contrast, zebrafish Etr-1 promoted skipping of both the NM and SM exons in a manner which was not dependent on URE-binding. CONCLUSIONS: Our results showed that Bruno-like proteins bind to UREs and regulate the alternative splicing of alpha-actinin pre-mRNA. Members of the Bruno family play multiple roles in splicing regulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping