PUBLICATION
Molecular cloning and developmental expression of zinc finger transcription factor MTF-1 gene in zebrafish, Danio rerio
- Authors
- Chen, W.-Y., John, A.C.J., Lin, C.-H., and Chang, C.-Y.
- ID
- ZDB-PUB-020306-3
- Date
- 2002
- Source
- Biochemical and Biophysical Research Communications 291(4): 798-805 (Journal)
- Registered Authors
- Chang, Chi-Yao
- Keywords
- MTF-1; zinc finger protein; expression; zebrafish; RT-PCR; whole mount in situ hybridization; embryonic development; hatching gland; eye; brain
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA-Binding Proteins
- Humans
- In Situ Hybridization
- Kinetics
- Molecular Sequence Data
- Phylogeny
- RNA, Messenger/biosynthesis
- Sequence Alignment
- Sequence Analysis, Protein
- Transcription Factors/biosynthesis*
- Transcription Factors/chemistry
- Transcription Factors/genetics*
- Zebrafish/embryology*
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zinc Fingers
- PubMed
- 11866436 Full text @ Biochem. Biophys. Res. Commun.
Citation
Chen, W.-Y., John, A.C.J., Lin, C.-H., and Chang, C.-Y. (2002) Molecular cloning and developmental expression of zinc finger transcription factor MTF-1 gene in zebrafish, Danio rerio. Biochemical and Biophysical Research Communications. 291(4):798-805.
Abstract
Metal-responsive transcription factor, MTF-1 is a zinc finger protein, shown to be essential for embryonic development. Homozygous knockout mouse embryos for MTF-1 die in utero at day 14 of gestation, due to liver decay. In the present study, we report the complete nucleotide sequence of cDNA encoding zebrafish MTF-1 and the amino acid sequence similarity with that of mouse, human, fish and Drosophila. The size of the zebrafish MTF-1 cDNA is 3,379 bp and the coding region (1,779 bp) encodes a polypeptide of 593 amino acids. The putative zinc finger and transactivation domains comprised by zebrafish MTF-1 were also determined. The zebrafish MTF-1 shows high identity of 97, 93, 93 and 67% in the DNA binding zinc finger domain and 51, 44, 48 and 20% overall identity with fugu, human, mouse and Drosophila, respectively. RT-PCR results show the maternal expression of MTF-1 transcripts. The pattern of MTF-1 gene expression during embryonic and early larval development was studied by whole-mount in situ hybridization using DIG-labeled anti-sense RNA probe. Stronger and ubiquitous expression was observed during the embryonic stages whereas, specific expression, especially in the neural parts, was observed throughout the stages studied after hatching.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping