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ZIRC
ZFIN ID: ZDB-PUB-020227-1
Forward and reverse genetic approaches to the analysis of eye development in zebrafish
Malicki, J.J., Pujic, Z., Thisse, C., Thisse, B., and Wei, X.
Date: 2002
Source: Vision Research 42(4): 527-533 (Journal)
Registered Authors: Malicki, Jarema, Pujic, Zac, Thisse, Bernard, Thisse, Christine, Wei, Xiangyun
Keywords: zebrafish; mutagenesis; screen; positional cloning; antisense; knockdown
MeSH Terms:
  • Animals
  • Eye/embryology*
  • Models, Animal*
  • Mutagenesis, Insertional
  • Mutagenesis, Site-Directed
  • Oligonucleotides, Antisense
  • Retinal Degeneration/genetics*
  • Sequence Analysis, DNA
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed: 11853769 Full text @ Vision Res.
ABSTRACT
The zebrafish has been established as a mainstream research system, largely due to the immense success of genetic screens. Over 2000 mutant alleles affecting zebrafish's early development have been isolated in two large-scale morphological screens and several smaller efforts. So far, over 50 mutant strains display retinal defects and many more have been shown to affect the retinotectal projection. More recently, mutant isolation and characterization have been successfully followed by candidate and positional cloning of underlying genes. To supplement forward genetic mutational analysis, several reverse genetic techniques have also been developed. These recent advances, combined with the genome project, have established the zebrafish as one of the leading models for studies of visual system development.
ADDITIONAL INFORMATIONNo data available