ZFIN ID: ZDB-PUB-020220-5
Isolation and expression of a novel neuron-specific onecut homeobox gene in zebrafish
Hong, S.K., Kim, C.-H., Yoo, K.-W., Kim, H.S., Kudoh, T., Dawid, I.B., and Huh, T.L.
Date: 2002
Source: Mechanisms of Development   112(1-2): 199-202 (Journal)
Registered Authors: Dawid, Igor B., Hong, Sung-Kook, Huh, Tae-Lin, Kim, Cheol-Hee, Kim, Hyung-Seok, Kudoh, Tetsuhiro, Yoo, Kyeong-Won
Keywords: zebrafish; onecut; homeodomain; brain; spinal cord; primary neurogenesis; mindbomb; narrowminded
MeSH Terms:
  • Animals
  • DNA, Complementary/metabolism
  • Drosophila
  • Drosophila Proteins
  • Homeodomain Proteins/biosynthesis*
  • Homeodomain Proteins/genetics*
  • In Situ Hybridization
  • Mesencephalon/embryology
  • Molecular Sequence Data
  • Nerve Tissue Proteins/biosynthesis
  • Neurons/metabolism*
  • Protein Structure, Tertiary
  • RNA, Messenger/metabolism
  • Rhombencephalon/embryology
  • Time Factors
  • Trans-Activators/biosynthesis*
  • Trans-Activators/genetics*
  • Zebrafish
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
PubMed: 11850195 Full text @ Mech. Dev.
A complete cDNA of a novel zebrafish gene named onecut has been isolated; this gene encodes a protein of 446 amino acids with a Cut domain (73 amino acid residues) and a homeodomain. The Cut domain of zebrafish Onecut is highly similar to those in mammalian hepatocyte nuclear factor-6 and Drosophila Onecut, sharing 90 and 88% amino acid identity, respectively. The expression of zebrafish onecut is restricted to neuronal cells, being first detected in trigeminal ganglia neurons at the end of gastrulation. By the 1-somite stage, onecut expression has begun in primary neurons of the lateral stripes in the neural plate, and appeared in neuronal cells of the medial stripes at the 2-somite stage. By the 4-somite stage, onecut expression expanded to the intermediate stripes and to subsets of neuronal cells in the midbrain and hindbrain. Subsequently, onecut expression intensified in the lateral region of midbrain and hindbrain, yet no onecut-positive cells were seen in the telencephalon. By 24hpf, onecut transcripts remained abundant in the spinal cord but were no longer detectable in differentiated Rohon-Beard sensory neurons. The expression of onecut was greatly increased in the neural mutant mindbomb, while being decreased in narrowminded.