PUBLICATION
Non-cell autonomous requirement for the bloodless gene in primitive hematopoiesis of zebrafish
- Authors
- Liao, E.C., Trede, N.S., Ransom, D., Zapata, A., Kieran, M., and Zon, L.I.
- ID
- ZDB-PUB-020220-13
- Date
- 2002
- Source
- Development (Cambridge, England) 129(3): 649-659 (Journal)
- Registered Authors
- Kieran, Mark, Liao, Eric, Ransom, David G., Trede, Nick, Zon, Leonard I.
- Keywords
- primitive hematopoiesis; embryonic; definitive; stem cell; zebrafish; bloodless; non-cell autonomous; scl; gatal
- MeSH Terms
-
- Anemia/genetics*
- Animals
- Antigens, Differentiation
- Apoptosis
- Basic Helix-Loop-Helix Transcription Factors
- Bone Marrow/embryology
- Bone Morphogenetic Protein 4
- Bone Morphogenetic Proteins/biosynthesis
- DNA-Binding Proteins/biosynthesis
- Erythroid-Specific DNA-Binding Factors
- GATA1 Transcription Factor
- Hematopoiesis/genetics*
- Lymphoid Tissue/embryology
- Mutation*
- Myeloid Cells
- Proto-Oncogene Proteins*
- Transcription Factors/biosynthesis
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 11830566 Full text @ Development
Citation
Liao, E.C., Trede, N.S., Ransom, D., Zapata, A., Kieran, M., and Zon, L.I. (2002) Non-cell autonomous requirement for the bloodless gene in primitive hematopoiesis of zebrafish. Development (Cambridge, England). 129(3):649-659.
Abstract
Vertebrate hematopoiesis occurs in two distinct phases, primitive (embryonic) and definitive (adult). Genes that are required specifically for the definitive program, or for both phases of hematopoiesis, have been described. However, a specific regulator of primitive hematopoiesis has yet to be reported. The zebrafish bloodless (bls) mutation causes absence of embryonic erythrocytes in a dominant but incompletely penetrant manner. Primitive macrophages appear to develop normally in bls mutants. Although the thymic epithelium forms normally in bls mutants, lymphoid precursors are absent. Nonetheless, the bloodless mutants can progress through embryogenesis, where red cells begin to accumulate after 5 days post-fertilization (dpf). Lymphocytes also begin to populate the thymic organs by 7.5 dpf. Expression analysis of hematopoietic genes suggests that formation of primitive hematopoietic precursors is deficient in bls mutants and those few blood precursors that are specified fail to differentiate and undergo apoptosis. Overexpression of scl, but not bmp4 or gata1, can lead to partial rescue of embryonic blood cells in bls. Cell transplantation experiments show that cells derived from bls mutant donors can differentiate into blood cells in a wild-type host, but wild-type donor cells fail to form blood in the mutant host. These observations demonstrate that the bls gene product is uniquely required in a non-cell autonomous manner for primitive hematopoiesis, potentially acting via regulation of scl.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping