ZFIN ID: ZDB-PUB-020218-5
Analysis of xanthophore and pterinosome biogenesis in zebrafish using methylene blue and pteridine autofluorescence
Le Guyader, S. and Jesuthasan, S.
Date: 2002
Source: Pigment cell research   15(1): 27-31 (Journal)
Registered Authors: Jesuthasan, Suresh, Le Guyader, Sylvie
Keywords: none
MeSH Terms:
  • Animals
  • Fluorescence
  • Light
  • Methylene Blue/metabolism
  • Pteridines/metabolism*
  • Pterins*
  • Ultraviolet Rays
  • Zebrafish/embryology*
PubMed: 11837453 Full text @ Pig. Cell Res.
ABSTRACT
We have identified two simple methods to analyse xanthophore and pterinosome biogenesis in zebrafish. The first uses methylene blue (methylthionium chloride), a redox dye which specifically labels xanthophores and pterinosomes, while the second uses autofluorescence to detect pteridine levels; these methods may be used to detect the number, location and shape of xanthophores and pterinosomes. These assays were applied to two zebrafish mutants--brie and yobo--and revealed that both mutants have pterinosome biogenesis and pteridine synthesis defects. Additionally, using capillary electrophoresis, we provide evidence that sepiapterin is responsible for the yellow colour and blue-light induced fluorescence in zebrafish embryos.
ADDITIONAL INFORMATION