|ZFIN ID: ZDB-PUB-020218-5|
Analysis of xanthophore and pterinosome biogenesis in zebrafish using methylene blue and pteridine autofluorescence
Le Guyader, S. and Jesuthasan, S.
|Source:||Pigment cell research 15(1): 27-31 (Journal)|
|Registered Authors:||Jesuthasan, Suresh, Le Guyader, Sylvie|
|PubMed:||11837453 Full text @ Pig. Cell Res.|
Le Guyader, S. and Jesuthasan, S. (2002) Analysis of xanthophore and pterinosome biogenesis in zebrafish using methylene blue and pteridine autofluorescence. Pigment cell research. 15(1):27-31.
ABSTRACTWe have identified two simple methods to analyse xanthophore and pterinosome biogenesis in zebrafish. The first uses methylene blue (methylthionium chloride), a redox dye which specifically labels xanthophores and pterinosomes, while the second uses autofluorescence to detect pteridine levels; these methods may be used to detect the number, location and shape of xanthophores and pterinosomes. These assays were applied to two zebrafish mutants--brie and yobo--and revealed that both mutants have pterinosome biogenesis and pteridine synthesis defects. Additionally, using capillary electrophoresis, we provide evidence that sepiapterin is responsible for the yellow colour and blue-light induced fluorescence in zebrafish embryos.