PUBLICATION
Bmp activity gradient regulates convergent extension during zebrafish gastrulation
- Authors
- Myers, D., Sepich, D., and Solnica-Krezel, L.
- ID
- ZDB-PUB-020128-4
- Date
- 2002
- Source
- Developmental Biology 243(1): 81-98 (Journal)
- Registered Authors
- Myers, Dina, Sepich, Diane, Solnica-Krezel, Lilianna
- Keywords
- convergence; extension; morphology; dorsoventral patterning; morphogenesis
- MeSH Terms
-
- Embryo, Nonmammalian/physiology
- Bone Morphogenetic Proteins/physiology*
- Animals
- Bone Morphogenetic Protein 7
- Zebrafish Proteins
- Gene Expression Regulation, Developmental/physiology*
- Gastrula/physiology*
- Transforming Growth Factor beta*
- Body Patterning/physiology*
- Cell Movement/physiology
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 11846479 Full text @ Dev. Biol.
Citation
Myers, D., Sepich, D., and Solnica-Krezel, L. (2002) Bmp activity gradient regulates convergent extension during zebrafish gastrulation. Developmental Biology. 243(1):81-98.
Abstract
During vertebrate gastrulation, a ventral to dorsal gradient of bone morphogenetic protein (Bmp) activity establishes cell fates. Concomitantly, convergent extension movements narrow germ layers mediolaterally while lengthening them anteroposteriorly. Here, by measuring movements of cell populations in vivo, we reveal the presence of three domains of convergent extension movements in zebrafish gastrula. Ventrally, convergence and extension movements are absent. Lateral cell populations converge and extend at increasing speed until they reach the dorsal domain where convergence speed slows but extension remains strong. Using dorsalized and ventralized mutants, we demonstrate that these domains are specified by the Bmp activity gradient. In vivo cell morphology and behavior analyses indicated that low levels of Bmp activity might promote extension with little convergence by allowing mediolateral cell elongation and dorsally biased intercalation. Further, single cell movement analyses revealed that the high ventral levels of Bmp activity promote epibolic migration of cells into the tailbud, increasing tail formation at the expense of head and trunk. We show that high Bmp activity limits convergence and extension by negatively regulating expression of the wnt11 (silberblick) and wnt5a (pipetail) genes, which are required for convergent extension but not cell fate specification. Therefore, during vertebrate gastrulation, a single gradient of Bmp activity, which specifies cell fates, also regulates the morphogenetic process of convergent extension.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping