PUBLICATION
Identification, genomic organization, and mRNA expression of LACTB, encoding a serine beta-lactamase-like protein with an amino-terminal transmembrane domain
- Authors
- Smith, T.S., Southan, C., Ellington, K., Campbell, D., Tew, D.G., and Debouck, C.
- ID
- ZDB-PUB-020128-3
- Date
- 2001
- Source
- Genomics 78(1-2): 12-14 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Gene Expression
- Membrane Proteins/genetics*
- Sequence Alignment
- Mitochondrial Proteins
- Chromosomes, Human, Pair 15/genetics
- DNA/chemistry
- DNA/genetics
- Female
- Blotting, Northern
- Molecular Sequence Data
- Ribosomal Proteins*
- Synteny
- beta-Lactamases
- Animals
- Sequence Analysis, DNA
- Chromosome Mapping
- Genes/genetics*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism*
- Male
- Exons
- Introns
- Amino Acid Sequence
- Humans
- Tissue Distribution
- Mice
- Chromosomes/genetics
- Sequence Homology, Amino Acid
- PubMed
- 11707067 Full text @ Genomics
Citation
Smith, T.S., Southan, C., Ellington, K., Campbell, D., Tew, D.G., and Debouck, C. (2001) Identification, genomic organization, and mRNA expression of LACTB, encoding a serine beta-lactamase-like protein with an amino-terminal transmembrane domain. Genomics. 78(1-2):12-14.
Abstract
Database searching with bacterial serine beta-lactamases identified mouse expressed sequence tags (ESTs) with significant similarity scores.The cloned mouse cDNA encodes a novel 551-amino-acid protein, LACTB, with a predicted amino-terminal transmembrane domain but no signal peptide. It contains an active site motif related to C-class beta-lactamases. Homologues were detected in sequence data from human, rat, cow, rabbit, pig, toad, zebrafish, and Caenorhabditis elegans, but not in Saccharomyces cerevisiae or Drosophila melanogaster. The genes were mapped to human chromosome 15q22.1 and mouse chromosome 9. Sequencing of a 14.7-kb fragment of mouse genomic DNA defined six exons. A virtual human cDNA and a 549-residue protein, predicted from unfinished genomic sequence, showed the same intron/exon structure. Northern blot analysis showed expression of the 2.3-kb mRNA predominantly in mouse liver and human skeletal muscle. This is the first reported vertebrate example of this microbial peptidase family.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping