PUBLICATION
Q/R RNA editing of the AMPA receptor subunit 2 (GRIA2) transcript evolves no later than the appearance of cartilaginous fishes
- Authors
- Kung, S.S., Chen, Y.C., Lin, W.H., Chen, C.C., and Chow, W.Y.
- ID
- ZDB-PUB-020123-11
- Date
- 2001
- Source
- FEBS letters 509(2): 277-281 (Journal)
- Registered Authors
- Keywords
- glutamine/arginine RNA editing, vertebrate, hagfish, zebrafish, GRIA2, glur-B, site, channels, organization, family, gene
- MeSH Terms
-
- Animals
- Arginine/genetics*
- Dogfish/genetics
- Evolution, Molecular*
- Glutamine/genetics*
- Hagfishes/genetics
- Nucleic Acid Conformation
- Protein Subunits
- RNA Editing*
- Rana catesbeiana/genetics
- Receptors, AMPA/genetics*
- Tetraodontiformes/genetics
- Tilapia/genetics
- Zebrafish/genetics
- PubMed
- 11741603 Full text @ FEBS Lett.
Citation
Kung, S.S., Chen, Y.C., Lin, W.H., Chen, C.C., and Chow, W.Y. (2001) Q/R RNA editing of the AMPA receptor subunit 2 (GRIA2) transcript evolves no later than the appearance of cartilaginous fishes. FEBS letters. 509(2):277-281.
Abstract
The amino acid, either a glutamine (Q) or an arginine (R), at the Q/R site of the pore-lining segment (M2) of a vertebrate AMPA receptor subunit critically influences the properties of the receptor. The R codon of the mammalian AMPA receptor subunit 2 (GRIA2) transcript is not coded by the chromosomal sequence, but is created by posttranscriptional RNA editing activities. On the other hand, the R codons of some teleost GRIA2 homologs are coded by chromosomal sequences. To elucidate the evolution of the utilization of Q/R RNA editing in modifying vertebrate GRIA2 transcripts, the GRIA2 genes of five fish species and an amphibian were studied. The putative hagfish GRIA2 homolog (hfGRIA2) encodes an R codon, whereas shark and bullfrog GRIA2 genes specify a Q codon at the genomic Q/R site. All gnathostoma GRIA2 genes possess an intron splitting the coding regions of M2 and the third hydrophobic region (M3). The intronic components required for Q/R RNA editing are preserved in all the Q-coding vertebrate GRIA2 genes but are absent from the R-coding GRIA2 genes. Interestingly, the hfGRIA2 is intronless, suggesting that hfGRIA2 is unlikely evolved from a Q/R editing-competent gene. Results of this study suggest that modification of GRIA2 transcripts by Q/R editing is most likely acquired after the separation of the Agnatha and Gnathostome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping