PUBLICATION
Expression pattern of the single-minded gene in zebrafish embryos
- Authors
- Wen, H.-J., Wang, Y., Chen, S.-H., and Hu, C.-H.
- ID
- ZDB-PUB-011220-2
- Date
- 2002
- Source
- Mechanisms of Development 110(1-2): 231-235 (Journal)
- Registered Authors
- Hu, Chin-Hwa
- Keywords
- Single-minded; sim; Zebrafish; Diencephalons; Mid-diencephalic boundary (MDB); Caudal diencephalons; Pharyngeal arches; Esophagus; Liver; Pancreas; Intestine
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Basic Helix-Loop-Helix Transcription Factors
- DNA, Complementary/genetics
- DNA-Binding Proteins/genetics*
- Drosophila Proteins
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- Larva/growth & development
- Larva/metabolism
- Molecular Sequence Data
- Nuclear Proteins/genetics*
- Organ Specificity
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/growth & development
- PubMed
- 11744389 Full text @ Mech. Dev.
Citation
Wen, H.-J., Wang, Y., Chen, S.-H., and Hu, C.-H. (2002) Expression pattern of the single-minded gene in zebrafish embryos. Mechanisms of Development. 110(1-2):231-235.
Abstract
Recently we isolated a homolog of the Drosophila single-minded (sim) gene from a zebrafish cDNA library. The 4380-bp of zebrafish sim cDNA encodes a polypeptide of 585 amino acids with strikingly conserved bHLH and PAS A/B domains in the amino-terminal region. During embryogenesis, sim mRNA appears in the animal hemisphere as early as 3 h post-fertilization and is expressed in a widespread pattern throughout the epiblast at the 75% epiboly stage. During the segmentation stage, sim mRNA is prominently expressed in the primordium of the hindbrain and appears as a transverse stripe in the epithelial layers of the mid-diencephalic boundary (MDB). During the pharyngula stage, sim is no longer expressed in the hindbrain, but continues to be expressed in the MDB and extends to the caudal diencephalon along the ventral midline. In addition, sim mRNA is prominent in the two pharyngeal arches. During the larval stage, sim mRNA is transcribed in the esophagus, liver, panc! reas, and intestine. In contrast, sim mRNA is no longer detectable in the forebrain after hatching. In adult fish, sim is widely expressed in brain, eyes, gill, heart, liver, and intestine.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping