PUBLICATION

Ikaros expression as a marker for lymphoid progenitors during zebrafish development

Authors
Willett, C.E., Kawasaki, H., Amemiya, C.T., Lin, S., and Steiner, L.A.
ID
ZDB-PUB-011217-15
Date
2001
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   222(4): 694-698 (Journal)
Registered Authors
Amemiya, Chris, Lin, Shuo, Steiner, Lisa, Willett, Catherine
Keywords
zebrafish, Ikaros, hematopoiesis, thymus, mutations, DNA binding proteins, transcription factor, hematopoietic cells, gene encodes, defects, family, mouse, splanchnopleura, cloche, fetal
MeSH Terms
  • Animals
  • Aorta/embryology
  • Biomarkers
  • DNA-Binding Proteins*
  • Embryo, Nonmammalian/physiology
  • Gene Expression*
  • Ikaros Transcription Factor
  • Kidney/embryology
  • Lymphoid Tissue/cytology*
  • Mesoderm/cytology
  • Mesoderm/metabolism
  • Pharynx/embryology
  • Stem Cells/cytology*
  • Thymus Gland/embryology
  • Tissue Distribution
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins
PubMed
11748838 Full text @ Dev. Dyn.
Abstract
The Ikaros gene encodes a transcription factor that, in mice, has been shown to be essential for the correct differentiation of B and T lymphocytes and is expressed in all cells of the lymphoid lineage, including pluripotent hematopoietic stem cells. During development in zebrafish, Ikaros expression begins in lateral mesoderm, and continues in the intermediate cell mass (ICM), which is derived from lateral mesoderm and has been shown to generate primitive hematopoietic precursors. Cells expressing Ikaros were then seen on the ventral side of the dorsal aorta, known to be a location of definitive hematopoietic precursors in birds and mammals. Ikaros-expressing cells were also found in the pharyngeal region, near the forming thymus. Later, such cells were seen in the pronephros, the site of hematopoiesis in adult fish. The timing of appearance of Ikaros-expressing cells suggests that, similar to other vertebrates, lymphocytes in the thymus arise from hematopoietic tissue located near the dorsal aorta or in the ICM.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping