PUBLICATION
Brain-derived neurotrophic factor gene organization and transcription in the zebrafish embryo
- Authors
- Huynh, G. and Heinrich, G.
- ID
- ZDB-PUB-011116-1
- Date
- 2001
- Source
- International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 19(7): 663-673 (Journal)
- Registered Authors
- Heinrich, Gerhard, Huynh, Gigi
- Keywords
- zebrafish embryo; promoter; enhanced green fluorescent protein; neuron; reporter gene; transcription unit
- MeSH Terms
-
- Animals
- Binding Sites/genetics
- Blotting, Southern
- Body Patterning/genetics
- Brain-Derived Neurotrophic Factor/genetics*
- Cloning, Molecular
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Exons/genetics*
- Gene Expression Regulation, Developmental/genetics*
- Gene Library
- Genes, Reporter/genetics
- Molecular Sequence Data
- Polymorphism, Restriction Fragment Length
- Promoter Regions, Genetic/genetics*
- Restriction Mapping
- Sequence Homology, Nucleic Acid*
- Transcription, Genetic/genetics*
- Transgenes/genetics
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 11705671 Full text @ Int. J. Dev. Neurosci.
Citation
Huynh, G. and Heinrich, G. (2001) Brain-derived neurotrophic factor gene organization and transcription in the zebrafish embryo. International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 19(7):663-673.
Abstract
The gene encoding zebrafish brain-derived neurotrophic factor (BDNF) was cloned from a PAC genomic DNA library. The entire transcription unit was contained in two independently isolated clones that together encompass 120 kb of genomic DNA. The intron/exon organization of the zebrafish gene was found to be identical to that of the mammalian gene but only one promoter has so far been identified. The associated 5' exon is 67% identical to exon 1c of the rat BDNF gene. A search of the 5' flank of the cloned promoter for sequence similarities with known transcription factor binding sites revealed potential AP-1, CREB, and SP1 binding sites. Fusion constructs containing the cloned promoter and 1.7 kb of 5' flank and an enhanced green fluorescent protein reporter that becomes membrane-anchored were injected into 1-8 cell stage embryos. Expression was seen in notochord, muscle, epithelial and endothelial cells of the 1-day-old embryo in consonance with the endogenous gene. These results demonstrate that the cloned promoter mediates cell-specific expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping