PUBLICATION
A zebrafish nanos related gene is essential for the development of primordial germ cells
- Authors
- Köprunner, M., Thisse, C., Thisse, B., and Raz, E.
- ID
- ZDB-PUB-011109-1
- Date
- 2001
- Source
- Genes & Development 15(21): 2877-2885 (Journal)
- Registered Authors
- Köprunner, Marion, Raz, Erez, Thisse, Bernard, Thisse, Christine
- Keywords
- nanos; germ line; PGC; germ plasm; maternal RNA; cell migration; zebrafish
- MeSH Terms
-
- Time Factors
- RNA-Binding Proteins*
- Nitric Oxide Synthase Type I
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Sequence Homology, Amino Acid
- In Situ Hybridization
- Animals
- Insect Proteins/genetics*
- Insect Proteins/physiology*
- Cell Survival
- Zebrafish
- Germ Cells/metabolism*
- Nitric Oxide Synthase/genetics*
- Nitric Oxide Synthase/physiology*
- Cell Movement
- Gene Expression Regulation, Developmental
- Plasmids/metabolism
- RNA/metabolism
- Drosophila Proteins*
- Amino Acid Sequence
- Molecular Sequence Data
- PubMed
- 11691838 Full text @ Genes & Dev.
Citation
Köprunner, M., Thisse, C., Thisse, B., and Raz, E. (2001) A zebrafish nanos related gene is essential for the development of primordial germ cells. Genes & Development. 15(21):2877-2885.
Abstract
Asymmetrically distributed cytoplasmic determinants collectively termed germ plasm have been shown to play an essential role in the development of primordial germ cells (PGCs). Here, we report the identification of a nanos-like (nanos1) gene, which is expressed in the germ plasm and in the PGCs of the zebrafish. We find that several mechanisms act in concert to restrict the activity of Nanos1 to the germ cells including RNA localization and control over the stability and translatability of the RNA. Reducing the level of Nanos1 in zebrafish embryos revealed an essential role for the protein in ensuring proper migration and survival of PGCs in this vertebrate model organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping