PUBLICATION

Brain-derived neurotrophic factor and TrkB tyrosine kinase receptor gene expression in zebrafish embryo and larva

Authors
Lum, T., Huynh, G., and Heinrich, G.
ID
ZDB-PUB-011031-9
Date
2001
Source
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience   19(6): 569-587 (Journal)
Registered Authors
Heinrich, Gerhard, Huynh, Gigi
Keywords
TrkB; zebrafish embryo; zebrafish larva; BDNF
MeSH Terms
  • Aging/genetics
  • Animals
  • Blastocyst/cytology
  • Blastocyst/metabolism
  • Blastomeres/cytology
  • Blastomeres/metabolism
  • Brain-Derived Neurotrophic Factor/genetics*
  • Brain-Derived Neurotrophic Factor/metabolism
  • Cell Differentiation/genetics*
  • Cross Reactions/immunology
  • Embryo, Mammalian/cytology
  • Embryo, Mammalian/embryology
  • Embryo, Mammalian/metabolism
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental/physiology*
  • Immunohistochemistry
  • Larva/cytology
  • Larva/genetics
  • Larva/metabolism
  • Mammals/immunology
  • Nervous System/cytology
  • Nervous System/embryology*
  • Nervous System/metabolism
  • Protein Isoforms/genetics
  • Protein Structure, Tertiary/genetics
  • RNA, Messenger/metabolism*
  • Receptor, trkB/genetics*
  • Receptor, trkB/metabolism
  • Receptor, trkC/genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Somites/cytology
  • Somites/metabolism
  • Transcription, Genetic/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed
11600319 Full text @ Int. J. Dev. Neurosci.
Abstract
The genes that encode the neurotrophin family of secreted polypeptides and the Trk family of high affinity neurotrophin transmembrane protein tyrosine kinase receptors are induced at the time of neurogenesis in mammals and are known to play critical roles in nervous system development. We show here that in contrast to mammals, the genes encoding the neurotrophin brain-derived neurotrophic factor (BDNF) and the neurotrophin receptor TrkB are expressed throughout embryonic development in the zebrafish. At the embryonic stages preceding transcription of endogenous genes all cells contain BDNF transcripts and immunoreactive BDNF and the trkB transcripts lack the region that encodes a kinase domain. As development proceeds, progressively fewer cells contain BDNF transcripts and by the time of neurogenesis the trkB transcripts encode a kinase-domain. In the 4-day-old larva, a small subset of specialized sensory cells on the surface and cells in deeper structures including the gill arches, fin, and cloaca express the BDNF gene at high levels in a promoter-specific fashion. This progressive restriction of BDNF gene expression must involve an extinction of BDNF gene transcription in some and induction of high levels of transcription in a promoter-specific fashion in other cells.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping