PUBLICATION
Brain-derived neurotrophic factor and TrkB tyrosine kinase receptor gene expression in zebrafish embryo and larva
- Authors
- Lum, T., Huynh, G., and Heinrich, G.
- ID
- ZDB-PUB-011031-9
- Date
- 2001
- Source
- International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 19(6): 569-587 (Journal)
- Registered Authors
- Heinrich, Gerhard, Huynh, Gigi
- Keywords
- TrkB; zebrafish embryo; zebrafish larva; BDNF
- MeSH Terms
-
- Aging/genetics
- Animals
- Blastocyst/cytology
- Blastocyst/metabolism
- Blastomeres/cytology
- Blastomeres/metabolism
- Brain-Derived Neurotrophic Factor/genetics*
- Brain-Derived Neurotrophic Factor/metabolism
- Cell Differentiation/genetics*
- Cross Reactions/immunology
- Embryo, Mammalian/cytology
- Embryo, Mammalian/embryology
- Embryo, Mammalian/metabolism
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental/physiology*
- Immunohistochemistry
- Larva/cytology
- Larva/genetics
- Larva/metabolism
- Mammals/immunology
- Nervous System/cytology
- Nervous System/embryology*
- Nervous System/metabolism
- Protein Isoforms/genetics
- Protein Structure, Tertiary/genetics
- RNA, Messenger/metabolism*
- Receptor, trkB/genetics*
- Receptor, trkB/metabolism
- Receptor, trkC/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Somites/cytology
- Somites/metabolism
- Transcription, Genetic/physiology
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 11600319 Full text @ Int. J. Dev. Neurosci.
Citation
Lum, T., Huynh, G., and Heinrich, G. (2001) Brain-derived neurotrophic factor and TrkB tyrosine kinase receptor gene expression in zebrafish embryo and larva. International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 19(6):569-587.
Abstract
The genes that encode the neurotrophin family of secreted polypeptides and the Trk family of high affinity neurotrophin transmembrane protein tyrosine kinase receptors are induced at the time of neurogenesis in mammals and are known to play critical roles in nervous system development. We show here that in contrast to mammals, the genes encoding the neurotrophin brain-derived neurotrophic factor (BDNF) and the neurotrophin receptor TrkB are expressed throughout embryonic development in the zebrafish. At the embryonic stages preceding transcription of endogenous genes all cells contain BDNF transcripts and immunoreactive BDNF and the trkB transcripts lack the region that encodes a kinase domain. As development proceeds, progressively fewer cells contain BDNF transcripts and by the time of neurogenesis the trkB transcripts encode a kinase-domain. In the 4-day-old larva, a small subset of specialized sensory cells on the surface and cells in deeper structures including the gill arches, fin, and cloaca express the BDNF gene at high levels in a promoter-specific fashion. This progressive restriction of BDNF gene expression must involve an extinction of BDNF gene transcription in some and induction of high levels of transcription in a promoter-specific fashion in other cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping