PUBLICATION
The 5'-flanking regions of CYP19A1 and CYP19A2 in zebrafish
- Authors
- Kazeto, Y., Ijiri, S., Place, A.R., Zohar, Y., and Trant, J.M.
- ID
- ZDB-PUB-011031-7
- Date
- 2001
- Source
- Biochemical and Biophysical Research Communications 288(3): 503-508 (Journal)
- Registered Authors
- Zohar, Yonathan
- Keywords
- cytochrome P450 aromatase; CYP19; 5'-flanking region; zebrafish; ERE; AhR; PPAR; RXR
- MeSH Terms
-
- 5' Untranslated Regions/genetics*
- Animals
- Aromatase/genetics*
- Base Sequence
- DNA/analysis
- Molecular Sequence Data
- Transcription, Genetic
- Zebrafish/genetics*
- PubMed
- 11676471 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kazeto, Y., Ijiri, S., Place, A.R., Zohar, Y., and Trant, J.M. (2001) The 5'-flanking regions of CYP19A1 and CYP19A2 in zebrafish. Biochemical and Biophysical Research Communications. 288(3):503-508.
Abstract
This report describes the structure of the 5'-flanking regions of both the CYP19A1 and A2 genes that were isolated from the genome of the zebrafish (Danio rerio). Consensus sequences of three cAMP-responsive elements (CRE), an aryl hydrocarbon-responsive element (AhR/Arnt), a steroidogenic factor 1 (SF-1) site, and a TATA box were observed in the 5'-flanking region of CYP19A1. In contrast, the 5'-flanking region of CYP19A2 was located upstream of an untranslated exon and possessed consensus sequences of a single CRE, an estrogen-responsive element (ERE), a peroxisome proliferator-activated receptor alpha/retinoid X receptor alpha heterodimer-responsive element (PPARalpha/RXRalpha), and a TATA box. Primer extension analysis revealed that the predominant transcription initiation sites for CYP19A1 and A2 transcripts were 28 and 91 bp upstream from the putative translation initiation codon, respectively. These analyses indicate that substantially different regulators, including a variety of environmental xenobiotics, control the expression the two CYP19 genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping