ZFIN ID: ZDB-PUB-010919-2
Behavioral screening for cocaine sensitivity in mutagenized zebrafish
Darland, T. and Dowling, J.E.
Date: 2001
Source: Proceedings of the National Academy of Sciences of the United States of America   98(20): 11691-11696 (Journal)
Registered Authors: Darland, Tristan, Dowling, John E.
Keywords: none
MeSH Terms:
  • Animals
  • Behavior, Animal/drug effects
  • Behavior, Animal/physiology*
  • Choice Behavior/drug effects
  • Choice Behavior/physiology*
  • Cocaine/pharmacology*
  • Dopamine Uptake Inhibitors/pharmacology*
  • Escape Reaction/physiology*
  • Learning/drug effects
  • Learning/physiology
  • Memory/drug effects
  • Memory/physiology
  • Mutagenesis*
  • Zebrafish/genetics
  • Zebrafish/physiology*
PubMed: 11553778 Full text @ Proc. Natl. Acad. Sci. USA
Understanding the molecular basis of addiction could be greatly aided by using forward genetic manipulation to lengthen the list of candidate genes involved in this complex process. Here, we report that zebrafish exhibit cocaine-induced conditioned place preference. In a pilot screen of 18 F(2) generation families of mutagenized fish, we found three with abnormally low responses to cocaine. This behavior was inherited by the F(3) generation in a manner that suggests the abnormalities were because of dominant mutations in single genes. Performance profiles in secondary behavioral screens measuring visual dark-adaptation and learning suggest that the defects were the result of mutations in distinct genes that affect dopaminergic signaling in the retina and brain.