PUBLICATION

Characterization of hrg22, a human homologue of the putative tumor suppressor gene hic1

Authors
Deltour, S., Pinte, S., Guerardel, C., and Leprince, D.
ID
ZDB-PUB-010918-7
Date
2001
Source
Biochemical and Biophysical Research Communications   287(2): 427-434 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Genome, Human
  • Cell Nucleus/metabolism
  • Kruppel-Like Transcription Factors
  • Gene Expression/drug effects
  • Humans
  • DNA-Binding Proteins*
  • Zebrafish Proteins
  • Molecular Sequence Data
  • Transcription Factors/chemistry
  • Transcription Factors/genetics*
  • Carrier Proteins/genetics*
  • Zebrafish
  • Genes, Tumor Suppressor/genetics*
  • Protein Structure, Tertiary
  • Chromosome Mapping
  • Sequence Homology, Amino Acid
  • Amino Acid Sequence
  • Animals
  • Hydroxamic Acids/pharmacology
  • Chromosomes, Human, Pair 22*
  • Conserved Sequence
  • Tumor Suppressor Proteins*
  • Base Sequence
PubMed
11554746 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Database searches identified on chromosome 22q11.2, a region subject to translocations, an homologue of the HIC1 (hypermethylated in cancer) candidate tumor suppressor gene located at 17p13.3. This gene was termed HRG22 for HIC1-related gene on chromosome 22. We have characterized a new HRG22 upstream coding exon and defined the complete coding sequence of the human and zebrafish HRG22 genes. Alignment of the HRG22 and HIC1 proteins from various species revealed high sequence homology in their N-terminal BTB/POZ and five C-terminal C(2)H(2) zinc finger domains and highlighted a conserved GLDLSKK/R peptide in their middle region. The full-length HRG22 and HIC1 proteins colocalize onto nuclear dots and share several functional properties since their BTB/POZ domains heterodimerize and are autonomous transcriptional repression domain insensitive to Trichostatin A, a histone deacetylase (HDAC) inhibitor. Thus, HIC1 and HRG22 define a subgroup of BTB/POZ domains unable to recruit repressing complexes containing an HDAC activity.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping