PUBLICATION

The zebrafish glypican Knypek controls cell polarity during gastrulation movements of convergent extension

Authors
Topczewski, J., Sepich, D.S., Myers, D.C., Walker, C., Amores, A., Lele, Z., Hammerschmidt, M., Postlethwait, J., and Solnica-Krezel, L.
ID
ZDB-PUB-010904-1
Date
2001
Source
Developmental Cell   1(2): 251-264 (Journal)
Registered Authors
Amores, Angel, Hammerschmidt, Matthias, Lele, Zsolt, Myers, Dina, Postlethwait, John H., Sepich, Diane, Solnica-Krezel, Lilianna, Topczewski, Jacek, Walker, Charline
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Body Patterning
  • Cell Division
  • Cloning, Molecular
  • Cysteine/chemistry
  • Dose-Response Relationship, Drug
  • Gastrula/physiology*
  • Glycoproteins/metabolism
  • Heparan Sulfate Proteoglycans/genetics*
  • Heparan Sulfate Proteoglycans/physiology*
  • In Situ Hybridization
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Protein Binding
  • Protein Structure, Tertiary
  • RNA/metabolism
  • RNA, Messenger/metabolism
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Time Factors
  • Wnt Proteins
  • Zebrafish
  • Zebrafish Proteins*
PubMed
11702784 Full text @ Dev. Cell
Abstract
Mutations in the zebrafish knypek locus impair gastrulation movements of convergent extension that narrow embryonic body and elongate it from head to tail. We demonstrate that knypek regulates cellular movements but not cell fate specification. Convergent extension movement defects in knypek are associated with abnormal cell polarity, as mutant cells fail to elongate and align medio-laterally. Positional cloning reveals that knypek encodes a member of the glypican family of heparan sulfate proteoglycans. Double mutant and overexpression analyses show that Knypek potentiates Wnt11 signaling, mediating convergent extension. These studies provide experimental and genetic evidence that glypican Knypek acts during vertebrate gastrulation as a positive modulator of noncanonical Wnt signaling to establish polarized cell behaviors underlying convergent extension movements.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes