PUBLICATION
The zebrafish glypican Knypek controls cell polarity during gastrulation movements of convergent extension
- Authors
- Topczewski, J., Sepich, D.S., Myers, D.C., Walker, C., Amores, A., Lele, Z., Hammerschmidt, M., Postlethwait, J., and Solnica-Krezel, L.
- ID
- ZDB-PUB-010904-1
- Date
- 2001
- Source
- Developmental Cell 1(2): 251-264 (Journal)
- Registered Authors
- Amores, Angel, Hammerschmidt, Matthias, Lele, Zsolt, Myers, Dina, Postlethwait, John H., Sepich, Diane, Solnica-Krezel, Lilianna, Topczewski, Jacek, Walker, Charline
- Keywords
- none
- MeSH Terms
-
- Animals
- Zebrafish
- RNA, Messenger/metabolism
- Time Factors
- Zebrafish Proteins*
- Wnt Proteins
- Glycoproteins/metabolism
- In Situ Hybridization
- Cysteine/chemistry
- Cell Division
- Body Patterning
- Gastrula/physiology*
- Protein Structure, Tertiary
- Phenotype
- Models, Genetic
- Amino Acid Sequence
- Signal Transduction
- Molecular Sequence Data
- Mutation
- RNA/metabolism
- Protein Binding
- Cloning, Molecular
- Dose-Response Relationship, Drug
- Sequence Homology, Amino Acid
- Heparan Sulfate Proteoglycans/genetics*
- Heparan Sulfate Proteoglycans/physiology*
- PubMed
- 11702784 Full text @ Dev. Cell
Citation
Topczewski, J., Sepich, D.S., Myers, D.C., Walker, C., Amores, A., Lele, Z., Hammerschmidt, M., Postlethwait, J., and Solnica-Krezel, L. (2001) The zebrafish glypican Knypek controls cell polarity during gastrulation movements of convergent extension. Developmental Cell. 1(2):251-264.
Abstract
Mutations in the zebrafish knypek locus impair gastrulation movements of convergent extension that narrow embryonic body and elongate it from head to tail. We demonstrate that knypek regulates cellular movements but not cell fate specification. Convergent extension movement defects in knypek are associated with abnormal cell polarity, as mutant cells fail to elongate and align medio-laterally. Positional cloning reveals that knypek encodes a member of the glypican family of heparan sulfate proteoglycans. Double mutant and overexpression analyses show that Knypek potentiates Wnt11 signaling, mediating convergent extension. These studies provide experimental and genetic evidence that glypican Knypek acts during vertebrate gastrulation as a positive modulator of noncanonical Wnt signaling to establish polarized cell behaviors underlying convergent extension movements.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping