PUBLICATION
Paralytic zebrafish lacking acetylcholine receptors fail to localize rapsyn clusters to the synapse
- Authors
- Ono, F., Higashijima, S., Shcherbatko, A., Fetcho, J.R., and Brehm, P.
- ID
- ZDB-PUB-010807-22
- Date
- 2001
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 21(15): 5439-5448 (Journal)
- Registered Authors
- Fetcho, Joseph R., Higashijima, Shin-ichi
- Keywords
- zebrafish; mutant; neuromuscular junction; acetylcholine receptor; dihydropyridine receptor; rapsyn; clustering
- MeSH Terms
-
- Calcium Channels, L-Type/metabolism
- Protein Transport/physiology
- Bungarotoxins/pharmacology
- Zebrafish
- In Vitro Techniques
- Green Fluorescent Proteins
- Fluorescent Dyes
- Muscle Proteins/metabolism*
- Ion Channel Gating/drug effects
- Paralysis/physiopathology*
- Muscle Contraction/drug effects
- Synaptic Transmission/drug effects
- Action Potentials/drug effects
- Action Potentials/genetics
- Spinal Cord/physiopathology
- Caffeine/pharmacology
- Motor Neurons/drug effects
- Motor Neurons/metabolism
- Motor Neurons/pathology
- Patch-Clamp Techniques
- Acetylcholine/pharmacology
- Luminescent Proteins/biosynthesis
- Luminescent Proteins/genetics
- Mutation*
- Receptors, Cholinergic/deficiency*
- Receptors, Cholinergic/genetics
- Electric Stimulation
- Synapses/drug effects
- Synapses/metabolism*
- Synapses/pathology
- Animals
- Receptor Aggregation/physiology
- Calcium Channel Blockers/pharmacology
- PubMed
- 11466415 Full text @ J. Neurosci.
Citation
Ono, F., Higashijima, S., Shcherbatko, A., Fetcho, J.R., and Brehm, P. (2001) Paralytic zebrafish lacking acetylcholine receptors fail to localize rapsyn clusters to the synapse. The Journal of neuroscience : the official journal of the Society for Neuroscience. 21(15):5439-5448.
Abstract
Physiological analysis of two lines of paralytic mutant zebrafish, relaxed and sofa potato, reveals defects in distinct types of receptors in skeletal muscle. In sofa potato the paralysis results from failed synaptic transmission because of the absence of acetylcholine receptors, whereas relaxed mutants lack dihydropyridine receptor-mediated release of internal calcium in response to the muscle action potential. Synaptic structure and function appear normal in relaxed, showing that muscle paralysis per se does not impede proper synapse development. However, sofa potato mutants show incomplete development of the postsynaptic complex. Specifically, in the absence of ACh receptors, clusters of the receptor-aggregating protein rapsyn form in the extrasynaptic membrane but generally fail to localize to the subsynaptic region. Our results indicate that, although rapsyn molecules are capable of self-aggregation, interaction with ACh receptors is required for proper subsynaptic localization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping