PUBLICATION
Comparative analysis of the ETV6 gene in vertebrate genomes from pufferfish to human
- Authors
- Montpetit, A. and Sinnett, D.
- ID
- ZDB-PUB-010723-24
- Date
- 2001
- Source
- Oncogene 20(26): 3437-3442 (Journal)
- Registered Authors
- Keywords
- ETV6, transcription factor, comparative genetics, vertebrate genomes, Fugu rubripes, chronic myelomonocytic leukemia, acute lymphoblastic leukemia, tyrosine kinase, fusion genes, tel, sequence, protein, mice, transformation
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Chickens/genetics
- DNA-Binding Proteins/genetics*
- Evolution, Molecular
- Fishes, Poisonous/genetics*
- Genes*
- Humans
- Mice
- Molecular Sequence Data
- Open Reading Frames
- Proto-Oncogene Proteins c-ets
- Repressor Proteins*
- Sequence Alignment
- Sequence Homology
- Species Specificity
- Transcription Factors/genetics*
- Vertebrates/genetics*
- Zebrafish/genetics
- PubMed
- 11423994 Full text @ Oncogene
Citation
Montpetit, A. and Sinnett, D. (2001) Comparative analysis of the ETV6 gene in vertebrate genomes from pufferfish to human. Oncogene. 20(26):3437-3442.
Abstract
The ETV6 gene encodes an Ets-like transcription factor that is frequently rearranged in leukemias. While some of the functions of ETV6 have been uncovered recently, little is known about the key structural elements involved. Comparative genome analysis may provide novel insights into gene evolution and
>functions. In this study, me cloned and sequenced the homologue of ETV6 from the compact genome of the pufferfish Fugu rubripes (fETV6). The genomic structure of the fETV6 gene was investigated by sequence analysis of a contig of genomic clones. The fETV6 gene, composed of eight exons, spans about 15 kb and is 16 times smaller than its human counterpart mainly because of the
>reduced intron size. Three of the seven introns of fETV are unusually large (more than 2 kb), including the 8.2 kb intron 2. The gene codes for a protein of 465 amino acids that is highly related to its human homologue, exhibiting an overall identity of 58% (72% similarity). To investigate the functional and evolutionary aspects of ETV6, we undertook a comparative analysis of this gene from various vertebrates (human, mouse, chicken, zebrafish and Fugu). As expected, the PNT and ETS domains were highly conserved, with on average 81 and 95% peptide sequence identity, respectively. In addition, we found several new highly conserved regions within the central section of the protein that are likely to represent further functional or structural domains, which may be associated with the transcription repression capacity of this protein. We also found conserved putative regulatory elements in the promoter as well as in the large intron 2 of fETV6. The information derived from this comparative analysis will serve as the basis for more precise functional studies of ETV6 gene regulation and function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping