PUBLICATION

Analysis of pancreatic development in living transgenic zebrafish embryos

Authors
Huang, H., Vogel, S.S., Liu, N., Melton, D.A., and Lin, S.
ID
ZDB-PUB-010601-3
Date
2001
Source
Molecular and Cellular Endocrinology   177(1-2): 117-124 (Journal)
Registered Authors
Huang, Haigen, Lin, Shuo, Liu, Ningai, Melton, Douglas A.
Keywords
zebrafish; pancreas; Pdx-1; insulin; transgenic
MeSH Terms
  • 5' Untranslated Regions
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/metabolism
  • Embryo, Nonmammalian/anatomy & histology
  • Embryo, Nonmammalian/chemistry
  • Green Fluorescent Proteins
  • Homeodomain Proteins*
  • Insulin/genetics
  • Insulin/metabolism
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Organ Specificity
  • Pancreas/embryology
  • Pancreas/growth & development*
  • Time Factors
  • Trans-Activators/metabolism
  • Zebrafish/embryology*
PubMed
11377827 Full text @ Mol. Cell. Endocrinol.
Abstract
Using DNA constructs containing regulatory sequences of the zebrafish Pdx-1 and insulin genes, germline transgenic zebrafish expressing the green fluorescent protein (GFP) reporter gene in the pancreas were generated. For both constructs, the GFP expression patterns in transgenic embryos were consistent with the mRNA expression patterns detected by RNA in situ hybridization. A deletion promoter analysis revealed that positive and negative cis-acting elements were involved in regulation of insulin gene expression. Three-dimensional reconstructions imaged from living embryos using two-photon laser-scanning microscopy (TPLSM) demonstrated that the zebrafish pancreas is formed from a single dorsal pancreatic cell mass. This is in contrast to mammals where the pancreas derives from both dorsal and ventral anlage. These transgenic fish should be useful for in vivo studies of factors involved in specifying and regulating pancreatic development and function.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping