PUBLICATION
Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element
- Authors
- Molina, A., Di Martino, E., Martial, J.A., and Muller, M.
- ID
- ZDB-PUB-010601-13
- Date
- 2001
- Source
- The Biochemical journal 356(2): 353-359 (Journal)
- Registered Authors
- Martial, Joseph A., Molina, Alfredo, Muller, Marc
- Keywords
- HSP70; transcription; zebrafish
- MeSH Terms
-
- Animals
- Base Sequence
- Binding Sites/genetics
- Carps/genetics
- Cells, Cultured
- DNA/genetics
- DNA/metabolism
- DNA Primers/genetics
- Gene Expression
- Genes, Reporter
- HSP70 Heat-Shock Proteins/genetics*
- Hot Temperature
- Mutagenesis, Site-Directed
- Promoter Regions, Genetic*
- Sequence Homology, Amino Acid
- Tilapia/genetics*
- Transcription Factors/metabolism
- Zebrafish/embryology
- Zebrafish/genetics
- PubMed
- 11368761 Full text @ Biochem. J.
Citation
Molina, A., Di Martino, E., Martial, J.A., and Muller, M. (2001) Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element. The Biochemical journal. 356(2):353-359.
Abstract
We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. -800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping