PUBLICATION

Sparking new frontiers: using in vivo electroporation for genetic manipulations

Authors
Swartz, M., Eberhart, J., Mastick, G.S., and Krull, C.E.
ID
ZDB-PUB-010514-4
Date
2001
Source
Developmental Biology   233(1): 13-21 (Review)
Registered Authors
Eberhart, Johann, Swartz, Mary
Keywords
none
MeSH Terms
  • Animals
  • Chick Embryo
  • Electroporation/methods*
  • Embryology/methods*
  • Genetic Techniques*
  • In Vitro Techniques
  • Mice
  • Nervous System/embryology
PubMed
11319854 Full text @ Dev. Biol.
Abstract
In vivo electroporation is a fascinating new approach by which gene expression, regulation, and function can be studied in developmental systems. This technique offers new opportunities for manipulations in animal models that lack genetic approaches, including avians. Furthermore, this approach is applicable to other embryo populations including mice, ascidians, zebrafish, Xenopus, and Drosophila. In this review, we discuss technical aspects of in vivo electroporation, review recent studies where this approach has been utilized successfully, and identify future directions.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping