PUBLICATION
Nodal signaling and the zebrafish organizer
- Authors
- Schier, A.F. and Talbot, W.S.
- ID
- ZDB-PUB-010410-3
- Date
- 2001
- Source
- The International journal of developmental biology 45(1): 289-97 (Journal)
- Registered Authors
- Schier, Alexander, Talbot, William S.
- Keywords
- none
- MeSH Terms
-
- Animals
- Body Patterning
- Genes, Homeobox
- Homeodomain Proteins/genetics
- Homeodomain Proteins/physiology
- Left-Right Determination Factors
- Mesoderm/cytology
- Mutation
- Nervous System/embryology
- Nodal Protein
- Organizers, Embryonic*
- Phenotype
- Signal Transduction
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology
- Zebrafish Proteins*
- PubMed
- 11291859
Citation
Schier, A.F. and Talbot, W.S. (2001) Nodal signaling and the zebrafish organizer. The International journal of developmental biology. 45(1):289-97.
Abstract
Systematic genetic screens in zebrafish have led to the discovery of mutations that affect organizer function and development. The molecular isolation and phenotypic analysis of the affected genes have revealed that TGF-beta signals of the Nodal family play a key role in organizer formation. The activity of the Nodal signals Cyclops and Squint is regulated extracellularly by the EGF-CFC cofactor One-eyed Pinhead and by antagonists belonging to the Lefty family of TGF-beta molecules. In the absence of Nodal signaling, the fate of cells in the organizer is transformed from dorsal mesoderm to neural ectoderm. Differential Nodal signaling also patterns the organizer along the anterior-posterior axis, with high levels required for anterior cell fates and lower levels for posterior fates. In addition, Nodal signaling cooperates with the homeodomain transcription factor Bozozok in organizer formation and neural patterning. The combination of genetic, molecular and embryological approaches in zebrafish has thus provided a framework to understand the mechanisms underlying organizer development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping