PUBLICATION

Regulation of dharma/bozozok by the Wnt Pathway

Authors

Ryu, S.-L., Fujii, R., Yamanaka, Y., Shimizu, T., Yabe, T., Hirata, T., Hibi, M., and Hirano, T.

ID

ZDB-PUB-010306-9

Date

2001

Source

Developmental Biology 231(2): 397-409 (Journal)

Registered Authors

Fujii, Ritsuko, Hibi, Masahiko, Hirata, Tsutomu, Ryu, Seung-Lim, Shimizu, Takashi, Yabe, Taijirou, Yamanaka, Yojiro

Keywords

dharma; bozozok; Wnt signal; Nieuwkoop center; ß-catenin; Tcf; Lef

MeSH Terms

Transforming Growth Factor beta/metabolism
Zebrafish Proteins*
Trans-Activators*
Down-Regulation
DNA/metabolism
Proto-Oncogene Proteins/genetics
Proto-Oncogene Proteins/physiology*
Time Factors
Luminescent Proteins/metabolism
RNA/metabolism
Green Fluorescent Proteins
Cytoskeletal Proteins/metabolism
DNA, Complementary/metabolism
Binding Sites
beta Catenin
RNA, Messenger/metabolism
Promoter Regions, Genetic
Signal Transduction
Mutagenesis, Site-Directed
In Situ Hybridization
Cytoplasm/metabolism
TCF Transcription Factors
Genes, Reporter
Base Sequence
Animals
Genes, Dominant
Transcription Factors/genetics
Transcription Factors/metabolism
Up-Regulation
Gene Library
DNA Primers/metabolism
Nodal Protein
Zebrafish
Luciferases/metabolism
Transcription Factor 7-Like 1 Protein
Molecular Sequence Data
Wnt Proteins
DNA-Binding Proteins/genetics
DNA-Binding Proteins/metabolism
Plasmids/metabolism
HMGB Proteins*
Lymphoid Enhancer-Binding Factor 1
Homeodomain Proteins/genetics
Homeodomain Proteins/metabolism*

(all 44)

PubMed

11237468 Full text @ Dev. Biol.

Abstract

The zebrafish homeobox gene dharma/bozozok (boz) is required for the formation and/or function of the Nieuwkoop center and the subsequent induction of the Spemann organizer. dharma is expressed soon after the midblastula transition in the dorsal blastomeres and the dorsal yolk syncytial layer (YSL). We found that the expression of dharma was upregulated or ectopically induced by misexpression of a Wnt protein and cytoplasmic components of the Wnt signaling pathway and downregulated by the expression of dominant-negative Tcf3. A 1.4-kbp fragment of the dharma promoter region contains consensus sequences for Tcf/Lef binding sites. This promoter region recapitulated the Wnt-dependent and dorsal dharma expression pattern when it was fused to luciferase or GFP. Deletion and point mutant analyses revealed that the Tcf/Lef binding sites were required to drive this expression pattern. These data established that dharma/boz functions between the dorsal determinants-mediated Wnt signals and the formation of the Nieuwkoop center.

Genes / Markers

Figures