PUBLICATION
Regulation of dharma/bozozok by the Wnt Pathway
- Authors
- Ryu, S.-L., Fujii, R., Yamanaka, Y., Shimizu, T., Yabe, T., Hirata, T., Hibi, M., and Hirano, T.
- ID
- ZDB-PUB-010306-9
- Date
- 2001
- Source
- Developmental Biology 231(2): 397-409 (Journal)
- Registered Authors
- Fujii, Ritsuko, Hibi, Masahiko, Hirata, Tsutomu, Ryu, Seung-Lim, Shimizu, Takashi, Yabe, Taijirou, Yamanaka, Yojiro
- Keywords
- dharma; bozozok; Wnt signal; Nieuwkoop center; ß-catenin; Tcf; Lef
- MeSH Terms
-
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/physiology*
- Transforming Growth Factor beta/metabolism
- Cytoplasm/metabolism
- TCF Transcription Factors
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Up-Regulation
- Genes, Dominant
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Mutagenesis, Site-Directed
- Wnt Proteins
- beta Catenin
- Base Sequence
- Molecular Sequence Data
- RNA, Messenger/metabolism
- DNA/metabolism
- Genes, Reporter
- Animals
- Nodal Protein
- Down-Regulation
- Plasmids/metabolism
- In Situ Hybridization
- Luminescent Proteins/metabolism
- Green Fluorescent Proteins
- Luciferases/metabolism
- Lymphoid Enhancer-Binding Factor 1
- DNA, Complementary/metabolism
- Transcription Factor 7-Like 1 Protein
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- Promoter Regions, Genetic
- Time Factors
- Zebrafish
- DNA Primers/metabolism
- HMGB Proteins*
- Gene Library
- Zebrafish Proteins*
- Cytoskeletal Proteins/metabolism
- Binding Sites
- RNA/metabolism
- Signal Transduction
- Trans-Activators*
- PubMed
- 11237468 Full text @ Dev. Biol.
Citation
Ryu, S.-L., Fujii, R., Yamanaka, Y., Shimizu, T., Yabe, T., Hirata, T., Hibi, M., and Hirano, T. (2001) Regulation of dharma/bozozok by the Wnt Pathway. Developmental Biology. 231(2):397-409.
Abstract
The zebrafish homeobox gene dharma/bozozok (boz) is required for the formation and/or function of the Nieuwkoop center and the subsequent induction of the Spemann organizer. dharma is expressed soon after the midblastula transition in the dorsal blastomeres and the dorsal yolk syncytial layer (YSL). We found that the expression of dharma was upregulated or ectopically induced by misexpression of a Wnt protein and cytoplasmic components of the Wnt signaling pathway and downregulated by the expression of dominant-negative Tcf3. A 1.4-kbp fragment of the dharma promoter region contains consensus sequences for Tcf/Lef binding sites. This promoter region recapitulated the Wnt-dependent and dorsal dharma expression pattern when it was fused to luciferase or GFP. Deletion and point mutant analyses revealed that the Tcf/Lef binding sites were required to drive this expression pattern. These data established that dharma/boz functions between the dorsal determinants-mediated Wnt signals and the formation of the Nieuwkoop center.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping