PUBLICATION
Isolation of gonadal mutations in adult zebrafish from a chemical mutagenesis screen
- Authors
- Bauer, M.P. and Goetz, F.W.
- ID
- ZDB-PUB-010220-7
- Date
- 2001
- Source
- Biology of reproduction 64(2): 548-554 (Journal)
- Registered Authors
- Bauer, Mike, Goetz, Frederick W.
- Keywords
- follicle; gamete biology; ovary; ovum; sperm; testis
- MeSH Terms
-
- Animals
- Drug Evaluation, Preclinical
- Ethylnitrosourea/toxicity
- Female
- Gonads/abnormalities*
- Gonads/pathology
- Male
- Mutagenesis/drug effects*
- Mutagens/toxicity*
- Mutation/genetics*
- Oocytes/drug effects
- Oocytes/pathology
- Ovarian Follicle/drug effects
- Ovarian Follicle/pathology
- Ovary/abnormalities
- Ovary/pathology
- Phenotype
- Spermatogenesis/drug effects
- Spermatogenesis/genetics
- Testis/abnormalities
- Testis/pathology
- Zebrafish/genetics*
- PubMed
- 11159357 Full text @ Biol. Reprod.
Citation
Bauer, M.P. and Goetz, F.W. (2001) Isolation of gonadal mutations in adult zebrafish from a chemical mutagenesis screen. Biology of reproduction. 64(2):548-554.
Abstract
A mutagenesis screen was conducted on zebrafish using N:-ethyl N:-nitrosourea as a mutagen and an F2 crossing scheme to obtain homozygous mutants in the F3 generation. Whole abdomens of 3-mo-old F3 zebrafish progeny were fixed and mass-embedded in paraffin blocks. Blocks were cut with a microtome to obtain cross-sections of the entire body cavity that included the ovaries and testes. Slides of the cross-sections were analyzed for alterations in gonadal structure and gametogenesis and were compared with gonads of wild-type fish. A total of 125 mutagenized genomes in 81 families were screened and 11 mutations were observed that produced visible phenotypes in only one sex per family. Male mutations included testes without mature sperm that contained either predominantly spermatocytes or spermatogonia. Female mutations included ovaries containing 1) degenerating oocytes surrounded by hypertrophied follicle walls or stroma, 2) extrafollicular tissue proliferation, 3) proliferating postovulatory follicle walls, and 4) large numbers of degenerating preovulatory and postovulatory oocytes. While past screens on zebrafish have concentrated on early developmental mutations, the results of this study demonstrate for the first time that mutagenesis can be used with zebrafish to study reproduction in adult animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping