PUBLICATION
Regulation of steroidogenesis in transgenic mice and zebrafish
- Authors
- Hu, M., Chiang, E.F., Tong, S., Lai, W., Hsu, N., Wang, L.C., and Chung, B.
- ID
- ZDB-PUB-010219-16
- Date
- 2001
- Source
- Molecular and Cellular Endocrinology 171(1-2): 9-14 (Journal)
- Registered Authors
- Chiang, Evelyn, Chung, Bon-chu
- Keywords
- embryogenesis; P450scc; side-chain cleavage enzyme; steroidogenesis; Y1 cells
- MeSH Terms
-
- Adrenal Glands/metabolism
- Animals
- Cholesterol Side-Chain Cleavage Enzyme/genetics
- Cyclic AMP/pharmacology
- Gene Expression Regulation*
- Gonads/metabolism
- Mice
- Mice, Transgenic/genetics*
- Models, Animal
- Pituitary Hormones/pharmacology
- Promoter Regions, Genetic
- Steroids/biosynthesis*
- Zebrafish/genetics*
- PubMed
- 11165005 Full text @ Mol. Cell. Endocrinol.
Citation
Hu, M., Chiang, E.F., Tong, S., Lai, W., Hsu, N., Wang, L.C., and Chung, B. (2001) Regulation of steroidogenesis in transgenic mice and zebrafish. Molecular and Cellular Endocrinology. 171(1-2):9-14.
Abstract
Steroid hormones are important physiological regulators in the body. Steroid hormones are mainly synthesized in the adrenal and gonads. Their synthesis is stimulated by pituitary hormones through cAMP as an intracellular mediator. The first and rate-limiting step for steroid biosynthesis is catalyzed by CYP11A1. Important regulatory elements for the control of the CYP11A1 gene expression have been characterized both in vitro and in vivo. The SF-1-binding sites are cis-acting elements controlling the basal and cAMP-stimulated gene expression. Our transgenic mouse studies showed that the 2.3kb promoter contains information controlling developmentally regulated gene expression. Finally, we present our results on the cloning of steroidogenic genes in zebrafish, a new model organism for genetic studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping