ZFIN ID: ZDB-PUB-010102-1
Kheper, a Novel ZFH/δEF1 Family Member, Regulates the Development of the Neuroectoderm of Zebrafish (Danio rerio)
Muraoka, O., Ichikawa, H., Shi, H., Okumura, S., Taira, E., Higuchi, H., Hirano, T., Hibi, M., and Miki, N.
Date: 2000
Source: Developmental Biology 228(1): 29-40 (Journal)
Registered Authors: Hibi, Masahiko, Higuchi, Hiroshi, Ichikawa, Hisashi, Miki, Naomasa, Muraoka, Osamu
Keywords: zebrafish; ZFH; dEF1; zinc-finger; homeodomain; transcription factor; ectoderm; mesoderm; CtBP; repressor
MeSH Terms:
  • Alcohol Oxidoreductases
  • Amino Acid Sequence
  • Animals
  • Bone Morphogenetic Proteins/antagonists & inhibitors
  • Bone Morphogenetic Proteins/metabolism
  • Central Nervous System/embryology*
  • Central Nervous System/metabolism
  • Cloning, Molecular
  • DNA-Binding Proteins/chemistry
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Drosophila Proteins
  • Ectoderm/metabolism*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins/chemistry
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • In Situ Hybridization
  • Mesoderm/metabolism
  • Microinjections
  • Molecular Sequence Data
  • Mutation
  • Nerve Tissue Proteins/chemistry
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Phenotype
  • Phosphoproteins/metabolism
  • Protein Binding
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Repressor Proteins/chemistry
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Sequence Alignment
  • Transcription Factors/chemistry
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zinc Fingers*
PubMed: 11087624 Full text @ Dev. Biol.
Kheper is a novel member of the ZFH (zinc-finger and homeodomain protein)/deltaEF1 family in zebrafish. kheper transcripts are first detected in the epiblast of the dorsal blastoderm margin at the early gastrula stage and kheper is expressed in nearly all the neuroectoderm at later stages. kheper expression was expanded in noggin RNA-injected embryos and also in swirl mutant embryos and was reduced in bmp4 RNA-injected embryos and chordino mutant embryos, suggesting that kheper acts downstream of the neural inducers Noggin and Chordino. Overexpression of Kheper elicited ectopic expansion of the neuroectoderm-specific genes fkd3, hoxa-1, and eng3, and the ectopic expression of hoxa-1 was not inhibited by BMP4 overexpression. Kheper interacted with the transcriptional corepressors CtBP1 and CtBP2. Overexpression of a Kheper mutant lacking the homeodomain or of a VP16-Kheper fusion protein disturbed the development of the neuroectoderm and head structures. These data underscore the role of Kheper in the development of the neuroectoderm and indicate that Kheper acts as a transcriptional repressor.