PUBLICATION
Effects of infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus infection on hematopoietic precursors of the zebrafish
- Authors
- LaPatra, S.E., Barone, L., Jones, G.R., and Zon, L.I.
- ID
- ZDB-PUB-001221-14
- Date
- 2000
- Source
- Blood cells, molecules & diseases 26(5): 445-452 (Journal)
- Registered Authors
- Zon, Leonard I.
- Keywords
- zebrafish; hematopoiesis; virus; anemia; fish
- MeSH Terms
-
- Animals
- Cell Line
- Erythroid Precursor Cells/virology
- Hematocrit
- Hematopoietic Stem Cells/virology*
- Infectious pancreatic necrosis virus*
- Kidney/virology
- Myeloid Progenitor Cells/virology
- RNA Virus Infections/blood
- RNA Virus Infections/virology*
- Rhabdoviridae*
- Time Factors
- Zebrafish/blood*
- Zebrafish/virology
- PubMed
- 11112382 Full text @ Blood Cells Mol. Dis.
Citation
LaPatra, S.E., Barone, L., Jones, G.R., and Zon, L.I. (2000) Effects of infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus infection on hematopoietic precursors of the zebrafish. Blood cells, molecules & diseases. 26(5):445-452.
Abstract
The zebrafish Danio rerio is a new model system for studying the genetics of hematopoiesis. To define naturally occurring viruses which could infect and replicate within hematopoietic precursors of the zebrafish, infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) were studied. Infection of whole fish with viral supernatants demonstrated infectious replicants for both viruses, indicating that the virus host range includes the zebrafish. In other species, infection with these viruses leads to prominent hematopoietic necrosis of the head kidney, the major site of adult hematopoiesis. We detected a transient toxicity of the virus to hematopoietic precursors and terminally differentiated red cells after viral infections. The kinetics of hematopoietic defects between IHNV and IPNV infection differed; fish infected with either virus, however, recovered by 6 days postinfection. In contrast to other fish infected with the virus, hematocrit did not change appreciably during this time. These studies are the first to demonstrate IHNV and IPNV infection of the zebrafish and reveal the potential for use of such viruses for gene transfer experiments to infect zebrafish hematopoietic cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping