PUBLICATION
Zebrafish nma is involved in TGFb family signaling
- Authors
- Tsang, M., Kim, R., De Caestecker, M.P., Kudoh, T., Roberts, A.B., and Dawid, I.B.
- ID
- ZDB-PUB-001213-4
- Date
- 2000
- Source
- Genesis (New York, N.Y. : 2000) 28(2): 47-57 (Journal)
- Registered Authors
- Dawid, Igor B., Kudoh, Tetsuhiro, Tsang, Michael
- Keywords
- nma; Bambi; BMP; patterning; development
- MeSH Terms
-
- RNA/metabolism
- Female
- Radiation Hybrid Mapping
- Molecular Sequence Data
- Gene Expression Regulation, Developmental/physiology*
- Amino Acid Sequence
- Animals
- Receptors, Transforming Growth Factor beta/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Mutation
- Sequence Homology, Amino Acid
- Embryo, Nonmammalian/metabolism
- Signal Transduction/physiology*
- Protein Serine-Threonine Kinases
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- In Situ Hybridization
- Activin Receptors, Type I/physiology*
- Microinjections
- Body Patterning
- Xenopus/embryology
- Xenopus/genetics
- Membrane Proteins/genetics
- Membrane Proteins/physiology*
- Bone Morphogenetic Proteins/physiology
- Xenopus Proteins*
- PubMed
- 11064421 Full text @ Genesis
Citation
Tsang, M., Kim, R., De Caestecker, M.P., Kudoh, T., Roberts, A.B., and Dawid, I.B. (2000) Zebrafish nma is involved in TGFb family signaling. Genesis (New York, N.Y. : 2000). 28(2):47-57.
Abstract
Summary: Bone morphogenetic proteins (BMP) are members of the TGFbeta superfamily of secreted factors with important regulatory functions during embryogenesis. We have isolated the zebrafish gene, nma, that encodes a protein with high sequence similarity to human NMA and Xenopus Bambi. It is also similar to TGFbeta type I serine/theronine kinase receptors in the extracellular ligand-binding domain but lacks a cytoplasmic kinase domain. During development, nma expression is similar to that of bmp2b and bmp4, and analysis in the dorsalized and ventralized zebrafish mutants swirl and chordino indicates that nma is regulated by BMP signaling. Overexpression of nma during zebrafish and Xenopus development resulted in phenotypes that appear to be based on inhibition of BMP signaling. Biochemically, NMA can associate with TGFbeta type II receptors and bind to TGFbeta ligand. We propose that nma is a BMP-regulated gene whose function is to attenuate BMP signaling during development through interactions with type II receptors and ligands. genesis 28:47-57, 2000.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping