PUBLICATION
Gene structure and amino acid sequence of Latimeria chalumnae (coelacanth) myelin DM20: phylogenetic relation of the fish
- Authors
- Tohyama, Y., Kasama-Yoshida, H., Sakuma, M., Kobayashi, Y., Cao, Y., Hasegawa, M., Kojima, H., Tamai, Y., Tanokura, M., and Kurihara, T.
- ID
- ZDB-PUB-001206-5
- Date
- 1999
- Source
- Neurochemical research 24(7): 867-873 (Journal)
- Registered Authors
- Hasegawa, Minoru
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Chickens
- Chromosome Walking
- Dogs
- Fishes/genetics*
- Humans
- Mice
- Molecular Sequence Data
- Myelin Proteolipid Protein/chemistry
- Myelin Proteolipid Protein/genetics*
- Nerve Tissue Proteins*
- Phylogeny
- Polymerase Chain Reaction/veterinary
- Sequence Alignment
- Sharks
- Xenopus
- PubMed
- 10403627 Full text @ Neurochem. Res.
Citation
Tohyama, Y., Kasama-Yoshida, H., Sakuma, M., Kobayashi, Y., Cao, Y., Hasegawa, M., Kojima, H., Tamai, Y., Tanokura, M., and Kurihara, T. (1999) Gene structure and amino acid sequence of Latimeria chalumnae (coelacanth) myelin DM20: phylogenetic relation of the fish. Neurochemical research. 24(7):867-873.
Abstract
The structure of Latimeria chalumnae (coelacanth) proteolipid protein/DM20 gene excluding exon 1 was determined, and the amino acid sequence of Latimeria DM20 corresponding to exons 2-7 was deduced. The nucleotide sequence of exon 3 suggests that only DM20 isoform is expressed in Latimeria. The structure of proteolipid protein/DM20 gene is well preserved among human, dog, mouse, and Latimeria. Southern blot analysis indicates that Latimeria DM20 gene is a single-copy gene. When the amino acid sequences of DM20 were compared among various species, Latimeria was more similar to tetrapods than other fishes including lungfish, confirming the previous finding by immunoreactivity (Waehneldt and Malotka 1989 J. Neurochem. 52:1941-1943). However, when phylogenetic trees were constructed from the DM20 sequences, lungfish was clearly the closest to tetrapods. Latimeria was situated outside of lungfish by the maximum likelihood method. The apparent similarity of Latimeria DM20 to tetrapod proteolipid protein/DM20 is explained by the slow amino acid substitution rate of Latimeria DM20.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping