PUBLICATION
Heart and gut chiralities are controlled independently from initial heart position in the developing zebrafish
- Authors
- Chin, A.J., Tsang, M., and Weinberg, E.S.
- ID
- ZDB-PUB-001205-15
- Date
- 2000
- Source
- Developmental Biology 227(2): 403-421 (Journal)
- Registered Authors
- Chin, Alvin J., Tsang, Michael, Weinberg, Eric
- Keywords
- left-right axis; heart looping; gut chirality; heterotaxy; zebrafish
- MeSH Terms
-
- Mesoderm/cytology
- Transforming Growth Factor beta/genetics
- Left-Right Determination Factors
- Humans
- Homeodomain Proteins/genetics
- Heart/embryology*
- Animals
- In Situ Hybridization
- Zebrafish Proteins*
- Digestive System/embryology*
- Models, Biological
- Transcription Factors/genetics
- Mutation
- Paired Box Transcription Factors
- Zebrafish/embryology*
- Zebrafish/genetics
- Body Patterning/genetics
- Gene Expression Regulation, Developmental
- Nuclear Proteins*
- PubMed
- 11071763 Full text @ Dev. Biol.
Citation
Chin, A.J., Tsang, M., and Weinberg, E.S. (2000) Heart and gut chiralities are controlled independently from initial heart position in the developing zebrafish. Developmental Biology. 227(2):403-421.
Abstract
A fundamental problem in developmental biology is how left-right (LR) asymmetry is generated, both on the whole organism level and at the level of an individual organ or structure. To investigate the relationship of organ sidedness to organ chirality, we examined 12 zebrafish mutants for initial heart tube position and later heart looping direction (chirality). Anomalous initial heart position was found in seven mutants, which also demonstrated loss of normal LR asymmetry in lateral plate mesoderm (LPM) antivin/lefty-1 and Pitx2 expression. Those with a relatively normal notochord (cyc(b16), din, and spt) displayed a predictive correlation between initial heart position and heart chirality, whereas initial heart position and heart chirality were independently randomized in those with a defective notochord (flh, boz, ntl, and mom). The predictability of heart chirality in spt, din, and b16 embryos, even in the absence of normal antivin/lefty-1 and Pitx2 expression, strongly suggests that heart chirality is controlled by a process distinct from that which controls appropriate left-sided LPM expression of antivin-Pitx2 signaling pathway molecules. In addition, there was correlation of initial heart position with gut chirality (and also between heart chirality and gut chirality) in the first class of mutants with normal notochord, but not in the second class, which appears to model human heterotaxy syndrome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping