|ZFIN ID: ZDB-PUB-001017-4|
Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor a in zebrafish
Haire, R.N., Rast, J.P., Litman, R.T., and Litman, G.W.
|Source:||Immunogenetics 51(11): 915-923 (Journal)|
|Registered Authors:||Haire, Robert, Litman, Gary W.|
|Keywords:||DNA sequence; developmental model; immunoglobulin diversity; short-primer PCR; teleost immunoglobulin|
|PubMed:||11003385 Full text @ Immunogenetics|
Haire, R.N., Rast, J.P., Litman, R.T., and Litman, G.W. (2000) Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor a in zebrafish. Immunogenetics. 51(11):915-923.
ABSTRACTThe zebrafish (Danio rerio) has become a significant model for understanding the developmental regulation of gene expression and holds considerable potential for characterizing the development of the immune system. Using a number of different approaches, including heterologous hybridization and short-primer PCR, cDNAs for three different classes of light-chain genes were identified and characterized. The zebrafish light chains are similar to trout type 1, trout type 2, and catfish type F, respectively. T-cell antigen receptor alpha (TCRalpha) was also identified and characterized. A high proportion of unusual transcripts including sterile transcripts, germline VJC transcripts, aberrant splice forms, and V-V transcripts were encountered in the immunoglobulin and TCR cDNAs examined. The light-chain and TCRalpha loci each consist of multiple families of V gene segments, apparent even from the small numbers of cDNAs of each isotype sequenced. The gene sequences reported provide an essential set of markers of both B- and T-cell lineages that will facilitate investigations of immune system development.
- Genes / Markers (18)