ZFIN ID: ZDB-PUB-001017-4
Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor a in zebrafish
Haire, R.N., Rast, J.P., Litman, R.T., and Litman, G.W.
Date: 2000
Source: Immunogenetics   51(11): 915-923 (Journal)
Registered Authors: Haire, Robert, Litman, Gary W.
Keywords: DNA sequence; developmental model; immunoglobulin diversity; short-primer PCR; teleost immunoglobulin
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Complementary
  • Genes, T-Cell Receptor alpha*
  • Immunoglobulin Constant Regions/classification
  • Immunoglobulin Constant Regions/genetics*
  • Immunoglobulin Isotypes/classification
  • Immunoglobulin Isotypes/genetics
  • Immunoglobulin Joining Region/classification
  • Immunoglobulin Joining Region/genetics*
  • Immunoglobulin Light Chains/classification
  • Immunoglobulin Light Chains/genetics*
  • Immunoglobulin Variable Region/classification
  • Immunoglobulin Variable Region/genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Zebrafish/genetics*
  • Zebrafish/immunology
PubMed: 11003385 Full text @ Immunogenetics
ABSTRACT
The zebrafish (Danio rerio) has become a significant model for understanding the developmental regulation of gene expression and holds considerable potential for characterizing the development of the immune system. Using a number of different approaches, including heterologous hybridization and short-primer PCR, cDNAs for three different classes of light-chain genes were identified and characterized. The zebrafish light chains are similar to trout type 1, trout type 2, and catfish type F, respectively. T-cell antigen receptor alpha (TCRalpha) was also identified and characterized. A high proportion of unusual transcripts including sterile transcripts, germline VJC transcripts, aberrant splice forms, and V-V transcripts were encountered in the immunoglobulin and TCR cDNAs examined. The light-chain and TCRalpha loci each consist of multiple families of V gene segments, apparent even from the small numbers of cDNAs of each isotype sequenced. The gene sequences reported provide an essential set of markers of both B- and T-cell lineages that will facilitate investigations of immune system development.
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