PUBLICATION
Midline signals regulate retinal neurogenesis in zebrafish
- Authors
- Masai, I., Stemple, D.L., Okamoto, H., and Wilson, S.W.
- ID
- ZDB-PUB-000922-3
- Date
- 2000
- Source
- Neuron 27(2): 251-263 (Journal)
- Registered Authors
- Masai, Ichiro, Okamoto, Hitoshi, Stemple, Derek L., Wilson, Steve
- Keywords
- none
- MeSH Terms
-
- Retina/cytology
- Retina/embryology*
- Retina/metabolism
- Transforming Growth Factor beta/metabolism
- Transforming Growth Factor beta/pharmacology
- Visual Pathways/cytology
- Visual Pathways/embryology
- Visual Pathways/metabolism
- In Vitro Techniques
- Nodal Protein
- Growth Substances*
- Signal Transduction/genetics
- Signal Transduction/physiology*
- Animals
- Embryonic Induction/genetics
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/genetics
- PAX2 Transcription Factor
- Mutation
- Cell Differentiation/genetics
- Organ Specificity/genetics
- Zebrafish/embryology*
- Zebrafish Proteins*
- DNA-Binding Proteins/biosynthesis*
- DNA-Binding Proteins/genetics
- Neurons/cytology*
- Neurons/metabolism
- Transcription Factors/biosynthesis
- PubMed
- 10985346 Full text @ Neuron
Citation
Masai, I., Stemple, D.L., Okamoto, H., and Wilson, S.W. (2000) Midline signals regulate retinal neurogenesis in zebrafish. Neuron. 27(2):251-263.
Abstract
In zebrafish, neuronal differentiation progresses across the retina in a pattern that is reminiscent of the neurogenic wave that sweeps across the developing eye in Drosophila. We show that expression of a zebrafish homolog of Drosophila atonal, ath5, sweeps across the eye predicting the wave of neuronal differentiation. By analyzing the regulation of ath5 expression, we have elucidated the mechanisms that regulate initiation and spread of neurogenesis in the retina. ath5 expression is lost in Nodal pathway mutant embryos lacking axial tissues that include the prechordal plate. A likely role for axial tissue is to induce optic stalk cells that subsequently regulate ath5 expression. Our results suggest that a series of inductive events, initiated from the prechordal plate and progressing from the optic stalks, regulates the spread of neuronal differentiation across the zebrafish retina.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping