PUBLICATION

The zebrafish floating head mutant demonstrates podocytes play an important role in directing glomerular differentiation

Authors
Majumdar, A. and Drummond, I.A.
ID
ZDB-PUB-000824-1
Date
2000
Source
Developmental Biology   222(1): 147-157 (Journal)
Registered Authors
Drummond, Iain, Majumdar, Arindam
Keywords
zebrafish; floating head; pronephros; glomerulus; podocyte; sclerotome
MeSH Terms
  • Animals
  • Cell Differentiation/genetics*
  • Embryo, Nonmammalian/cytology
  • Homeodomain Proteins/genetics*
  • Kidney Glomerulus/cytology*
  • Kidney Glomerulus/ultrastructure
  • Microscopy, Electron
  • Mutation*
  • Transcription Factors/genetics*
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed
10885753 Full text @ Dev. Biol.
Abstract
In zebrafish, the pronephric glomerulus occupies a midline position underneath the notochord and is vascularized through angiogenic capillary ingrowth from the dorsal aorta. The midline mutants floating head (flh), sonic you (syu), and you-too (yot) provide the opportunity to study glomerular differentiation in the absence of the notochord and vascularization from the dorsal aorta. In flh, syu, and yot mutants, glomeruli differentiate at ectopic lateral positions within the embryo and contain morphologically identifiable podocyte and endothelial cell types. In the absence of the dorsal aorta, endothelia from an alternate source are recruited by podocytes during glomerular vascularization to make functional glomeruli. Our results suggest that midline signals are required for proper glomerular morphogenesis but not for the differentiation of podocytes. Podocytes appear to play an important role in directing cellular recruitment events leading to glomerular differentiation. Furthermore, we find defects in sclerotomal development that correlate with defects in glomerular morphogenesis suggesting a possible link between the formation of these embryonic structures.
Genes / Markers
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Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes