PUBLICATION
Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)
- Authors
- Denovan-Wright, E.M., Pierce, M., and Wright, J.M.
- ID
- ZDB-PUB-000622-6
- Date
- 2000
- Source
- BBA Gene Structure and Expression 1492(1): 221-226 (Journal)
- Registered Authors
- Wright, Jonathan M.
- Keywords
- brain fatty acid binding protein; zebrafish; cDNA cloning; in situ hybridization; Northern analysis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Brain/physiology*
- Carrier Proteins/genetics*
- Cloning, Molecular
- DNA, Complementary/analysis
- Fatty Acid-Binding Proteins
- Gene Expression
- Humans
- In Situ Hybridization
- Molecular Sequence Data
- Neoplasm Proteins*
- Nerve Tissue Proteins*
- Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Tumor Suppressor Proteins*
- Zebrafish
- Zebrafish Proteins*
- PubMed
- 11004493 Full text @ BBA Gene Structure and Expression
Citation
Denovan-Wright, E.M., Pierce, M., and Wright, J.M. (2000) Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio). BBA Gene Structure and Expression. 1492(1):221-226.
Abstract
We have determined the nucleotide sequence for two cDNA clones coding for a fatty acid binding protein (FABP) from zebrafish (Danio rerio). Comparison of the sequence with GenBank entries revealed extensive amino acid identity between this zebrafish FABP and brain FABPs (B-FABP) from other species. The zebrafish B-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases BglII or EcoRI suggesting that a single copy of the B-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish B-FABP mRNA is approximately 850 nucleotides in length. In situ hybridization revealed that the B-FABP mRNA was expressed in the periventricular gray zone of the optic tectum of the adult zebrafish brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping