PUBLICATION

Ubiquitination and degradation of the zebrafish paired-like homeobox protein VSX-1

Authors
Kurtzman, A.L., Gregori, L., Haas, A.L., Schechter, N.
ID
ZDB-PUB-000622-11
Date
2000
Source
Journal of neurochemistry   75(1): 48-55 (Journal)
Registered Authors
Kurtzman, Aaron, Schechter, Nisson
Keywords
retina; bipolar cells; ubiquitin; Chx10; 26S proteasome
MeSH Terms
  • Adenosine Triphosphatases/metabolism
  • Animals
  • COS Cells
  • Cysteine Endopeptidases/metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Eye Proteins/genetics
  • Eye Proteins/metabolism*
  • Gene Expression
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • Multienzyme Complexes/metabolism
  • Proteasome Endopeptidase Complex
  • Protein Processing, Post-Translational
  • Rabbits
  • Reticulocytes/metabolism
  • Retina/growth & development
  • Transfection
  • Ubiquitins/metabolism*
  • Zebrafish*
  • Zebrafish Proteins*
PubMed
10854246 Full text @ J. Neurochem.
Abstract
:Vsx-1 is a paired-like : CVC homeobox protein dynamically expressed during zebrafish development. Previous results indicate that Vsx-1 influences bipolar cell differentiation and maintenance of these cells in the adult retina. To understand the developmental regulation of this transcription factor, we investigated ubiquitination as a possible posttranslational mechanism. In vitro, Vsx-1 was conjugated with multiple ubiquitin moieties. Proteasome inhibitors and added ubiquitin increased the accumulation of Vsx-1-ubiquitin(n) complexes and stabilized unmodified Vsx-1. Also, in transiently transfected COS-7 cells, Vsx-1 is ubiquitinated, and pulse-chase experiments show that Vsx-1 proteolysis occurs. Vsx-1 proteins with C-terminal deletions retained the capacity for initial modification by ubiquitin but lost the capacity for efficient chain elongation. These results show that Vsx-1 is a substrate of the ubiquitin/proteasome pathway and suggest that C-terminal sequences of Vsx-1 are critical for ubiquitin chain elongation. In addition, our findings suggest that ubiquitin-dependent proteolysis regulates Vsx-1 during zebrafish retinal development.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping