PUBLICATION
Analysis of chromosomal rearrangements induced by postmeiotic mutagenesis with ethylnitrosourea in zebrafish
- Authors
- Imai, Y., Feldman, B., Schier, A.F., and Talbot, W.S.
- ID
- ZDB-PUB-000505-7
- Date
- 2000
- Source
- Genetics 155(1): 261-272 (Journal)
- Registered Authors
- Feldman, Benjamin, Imai, Yoshiyuki, Schier, Alexander, Talbot, William S.
- Keywords
- none
- MeSH Terms
-
- Animals
- Ethylnitrosourea/pharmacology
- Gene Deletion
- Genes, Lethal
- Germ Cells/physiology
- Meiosis/physiology*
- Mutagenesis/drug effects
- Mutagens/pharmacology
- Translocation, Genetic
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 10790400 Full text @ Genetics
Citation
Imai, Y., Feldman, B., Schier, A.F., and Talbot, W.S. (2000) Analysis of chromosomal rearrangements induced by postmeiotic mutagenesis with ethylnitrosourea in zebrafish. Genetics. 155(1):261-272.
Abstract
Mutations identified in zebrafish genetic screens allow the dissection of a wide array of problems in vertebrate biology. Most screens have examined mutations induced by treatment of spermatogonial (premeiotic) cells with the chemical mutagen N-ethyl-N-nitrosourea (ENU). Treatment of postmeiotic gametes with ENU induces specific-locus mutations at a higher rate than premeiotic regimens, suggesting that postmeiotic mutagenesis protocols could be useful in some screening strategies. Whereas there is extensive evidence that ENU induces point mutations in premeiotic cells, the range of mutations induced in postmeiotic zebrafish germ cells has been less thoroughly characterized. Here we report the identification and analysis of five mutations induced by postmeiotic ENU treatment. One mutation, snh(st1), is a translocation involving linkage group (LG) 11 and LG 14. The other four mutations, oep(st2), kny(st3), Df(LG 13)(st4), and cyc(st5), are deletions, ranging in size from less than 3 cM to greater than 20 cM. These results show that germ cell stage is an important determinant of the type of mutations induced. The induction of chromosomal rearrangements may account for the elevated frequency of specific-locus mutations observed after treatment of postmeiotic gametes with ENU.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping