PUBLICATION
Detection of a spontaneous high expression of heat shock protein 70 in developing zebrafish (Danio rerio)
- Authors
- Yeh, F.L. and Hsu, T.
- ID
- ZDB-PUB-000505-2
- Date
- 2000
- Source
- Bioscience, biotechnology, and biochemistry 64(3): 592-595 (Journal)
- Registered Authors
- Keywords
- Danio rerio; development; heat shock element; heat shock factor; heat shock protein 70
- MeSH Terms
-
- Animals
- Female
- Gene Expression
- HSP70 Heat-Shock Proteins/genetics*
- Male
- Zebrafish
- PubMed
- 10803959 Full text @ Biosci. Biotechnol. Biochem.
Citation
Yeh, F.L. and Hsu, T. (2000) Detection of a spontaneous high expression of heat shock protein 70 in developing zebrafish (Danio rerio). Bioscience, biotechnology, and biochemistry. 64(3):592-595.
Abstract
A spontaneous high expression of heat shock protein 70 (HSP 70) was detected in zebrafish (Danio rerio) at early larval stage (84 h after fertilization), but the HSP 70 level was either low or barely detectable in 12, 24, 36, 60, and 108 h after fertilization. The extracts of zebrafish at 80 and 84 h after fertilization formed a clear protein-DNA complex with a probe containing heat shock elements (HSEs), suggesting that this spontaneous expression of HSP 70 may be turned on via the binding of stage-specific HSE-binding factors to HSP 70 gene promotor. The protein-HSE complexes produced by the spontaneous binding, however, were found to be different from those formed by the extracts of heat-treated zebrafish in electrophoretic mobility.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping